GC-MS法研究矿物油在肉鸡体内残留动态变化  

GC-MS Investigation of Dynamic Changes of Mineral Oil Components in Broilers

作　　者：刘亮[1,2] 唐善虎[1] 陈诺[1] 刘内生[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]川北医学院实验动物中心,四川南充637100

出　　处：《食品科学》2010年第19期87-91,共5页Food Science

基　　金：西南民族大学研究项目

摘　　要：在动物生产防疫中常常使用以矿物油作为佐剂成分的动物疫苗免疫食用动物,这种处理有可能导致矿物油残留问题。本研究通过用矿物油注射15日龄雌性雏鸡模拟疫苗接种,探讨鸡体组织中矿物油成分残留的变化情况。实验中采用GC-MS法分别对15日龄肉鸡注射矿物油后3、7、14、28d和56d时鸡腿部肌肉、鸡心脏、肝和皮肤中矿物油成分进行检测。实验发现:注射矿物油后3d或7d,注射部位肌肉中仍然可以检测出矿物油阳性对照中的11种烷烃组分,且各组分平均相对百分含量与阳性对照趋势大致相同;在14d时注射部位残留烷烃的种类与数量都有下降趋势,56d时检出种类到达最少。心脏所含烷烃种类在注射后3d时最多,7d时检出的种类数减少;但与皮肤相比,心脏56d时所能检测到的矿物油成分种类数量都比较多。结果表明:矿物油会随机体循环到达其他组织器官,使用矿物油作为佐剂会给动物性食品生产带来一定的安全隐患。For animal disease control,vaccines containing mineral oil as a vaccine adjuvant composition are often used to immunize animals,but this may cause residues in animal meat,which will cause health problems to the human body once ingested. The objectives of this study were to determine the residues of mineral oil components in the chicken＇s body and to investigate the metabolic changes of mineral oil components in a simulated vaccination model. Chickens of 15 days old were injected with white oil No. 7 （a commercially available mineral oil adjuvant） and the residues of mineral oil components in leg muscle,heart,liver and skin were determined using GC-MS method on the 3rd,7th,14th,28th and 56th day after injection. It was found that on both 3rd and 7th day after injection 11 alkanes detected in white oil No. 7 were also detected in the muscle receiving injection,and each of these compounds had similar mean relative percentage in the two analytes; on the 14th day,both the number and quantity of residual alkanes in the injection site had a decrease trend,and on the 56th day reached minimum. While for both heart and liver,the number of detected alkanes was found maximum on the 3rd day,and became smaller on the 7th day,but compared with skin,much more number and quantity of residual alkanes were both determined in heart on the 56th day. These results show that mineral oil reach other tissues and organs via circulation,and using mineral oil as an adjuvant in food production of animal origin might bring some safety risks.

关 键 词：鸡肉 GC-MS 矿物油 残留 食品安全 

分 类 号：TS201.6[轻工技术与工程—食品科学]