荧光淬灭法测定ε-聚赖氨酸含量的研究  

Determination of ε-Polylysine Content by Fluorescence Quenching Method

作　　者：刘双燕[1] 贾士儒[1] 王国良[1] 戴玉杰[1] 谭之磊[1] 宋庆超[1] 

机构地区：[1]天津科技大学工业微生物教育部重点实验室,天津300457

出　　处：《现代食品科技》2010年第11期1267-1270,共4页Modern Food Science and Technology

基　　金：国家"973"项目(2007CB714305)

摘　　要：以异硫氰酸酯(FITC)为指示剂,采用荧光法测定ε-聚赖氨酸(ε-PL)。体系的激发波长和发射波长分别为495nm和519nm。在2%乙醇介质中,ε-PL与FITC反应,使FITC发生荧光淬灭。ε-PL含量在0.1～0.8mg/L范围内呈现一定的线性关系。本文探讨了ε-PL淬灭FITC的机理,并用荧光淬灭法检测了米饭样品中ε-PL的含量。结果表明,所测得的ε-PL含量与实际添加量较为接近。A new highly sensitive and selective fluorometric method has been developed for the determination of ε-PL using fluorescein isothiocyanate as fluorogenic reagent（λex=495 nm,λem=519 nm）.In 2% ethanol medium,ε-PL can make fluorescence isothiocyanate quenched.The linear range was 0.1-0.8mg/L.In this study,the fluorescence quenching mechanism of ε-PL to FITC was discussed,and the method has been applied to the determination of ε-PL added into rice with satisfactory results.

关 键 词：异硫氰酸酯 荧光淬灭法 Ε-聚赖氨酸 

分 类 号：TS207.3[轻工技术与工程—食品科学]