小型发酵罐条件下树干毕赤酵母木糖发酵条件优化  

Optimization of ethanol fermentation conditions with xylose by Pichia stipitis in bioreactor

作　　者：杨盛茹[1] 丁长河[1] 惠明[2] 张雪[3] 王向向[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450052 [2]河南工业大学生物工程学院,河南郑州450001 [3]郑州牧业工程高等专科学校食品工程系,河南郑州450011

出　　处：《中国酿造》2010年第12期65-68,共4页China Brewing

基　　金：河南省高校科技创新人才支持计划项目(101HASYTIT035)

摘　　要：以树干毕赤酵母m4为发酵木糖菌株,研究了5L发酵罐中不同培养条件对酵母发酵木糖生成乙醇和酵母生长规律的影响。结果表明,5L发酵罐中最佳发酵条件为:初始木糖浓度108g/L,温度28℃,通气量30L/h,转速300r/min,接种量12.5%,最适pH值为4.8,此时木糖转化率、乙醇得率最高,分别为98.5%,0.47g/g,乙醇产量有较大提高,50.0g/L。The effects of the different incubation conditions on the ethanol fermentation with xylose by Pichia stipitis m4 were studied. Growth of pattern of yeast and production of ethanol under different conditions in a 5L bioreactor were investigated. The results indicated that the optimum fermen- tation conditions in a 5L bioreactor were as follows： initial comem of xylose 108g/L, fermentation temperature at 28℃, ventilation level at 30L/h, the rotating speed 300r/min, inoculum 12.5%, pH 4.8. Under the optimum condition, rate of conversion of xylose to ethanol and yield of ethanol were 98.5% and 0.47g/g, respectively. Yield of ethanol significantly increased to 50.0g/L.

关 键 词：树干毕赤酵母 木糖 乙醇 发酵罐 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]