食品中非O157大肠杆菌志贺毒素基因序列分析  

Sequence Analysis of Shiga Toxin Gene from Non-O157 Escherichia coli Strain Isolated from Foods

作　　者：李睿[1] 戴诗皎[1] 戴锴[1] 杜德龙[1] 朱廷恒[2] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023 [2]浙江工业大学生物与环境工程学院,浙江杭州310000

出　　处：《食品科学》2010年第21期236-238,共3页Food Science

基　　金：湖北省自然科学基金重点项目(2009CDA118)

摘　　要：对一株从食品中分离的非O157产志贺毒素1型大肠杆菌(EC6)进行研究。将该菌株所产志贺毒素Stx1用PCR扩增stx1基因全长并克隆测序,其stx1基因与GenBank数据库收录的stx1基因最高同源性为99%,表明EC6发生了一定程度的基因突变。采用邻位相连法构建进化树,结果表明EC6为stx1基因亚型。A non-O157 E.coli strain（named EC6） which produces Shiga toxin type 1 was isolated from beef and the sequence of its Shiga toxin gene was analyzed.The full length of stx1 gene from strain EC6 was amplified by PCR,cloned into the pGEM-T Easy vector and sequenced.The results showed that the stx1 gene of EC6 had up to 99% similarity with the stx1 sequences deposited in GenBank database,indicating the occurrence of mutation.Furthermore,a phylogenetic tree was constructed by neighborjoining method,and according to it the stx1 gene of EC6 was stx1 genotype.

关 键 词：产志贺毒素大肠杆菌 食品 stx1基因 序列分析 非O157血清型 

分 类 号：R155.5[医药卫生—营养与食品卫生学]