生物富硒对沼泽红假单胞菌G3菌株营养组分的影响  

Effect of Selenium Enrichment on Nutritional Composition of Rhodopseudomonas palustris G3

作　　者：杨启银[1,2,3] 刘淑超[1] 贾秉晟[1] 王家芳[1] 曩潇潇 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]南京市微生物工程技术研究中心,江苏南京210046 [3]江苏省微生物资源工程化技术研究中心,江苏南京210046 [4]南京红太阳集团,江苏南京210031

出　　处：《食品科学》2010年第23期253-257,共5页Food Science

基　　金：江苏省科学技术厅高技术研究计划项目(BG2005326)

摘　　要：为了探讨生物富硒对沼泽红假单胞菌的营养品质的影响,对富硒沼泽红假单胞菌G3菌株和普通沼泽红假单胞菌的一般营养成分、氨基酸、B族维生素、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)进行测定。结果表明:富硒培养的沼泽红假单胞菌总硒含量为875.03μg/g,其中有机硒含量为847.42μg/g,有机硒转化率为96.85%。富硒沼泽红假单胞菌G3菌株的蛋白质、菌体可溶性糖和粗脂肪的含量分别比普通沼泽红假单胞菌提高了9.10%、5.13%和42.86%,灰分含量则比普通菌降低了23.73%。富硒沼泽红假单胞菌菌体含有17种氨基酸,普通菌体含有16种氨基酸,富硒沼泽红假单胞菌菌体的多种氨基酸含量均高于普通菌,其中人体必需的蛋氨酸和赖氨酸分别比对照提高了100%和9.2%。富硒沼泽红假单胞菌的VB2、VB6、烟酸和类胡萝卜素与对照组相比均明显提高,VB2的含量提高了6.45%,VB6的含量提高了5.77%,烟酸的含量提高了7.20%,类胡萝卜素含量提高了45.08%,VB1与对照组相比下降了9.2%,富硒沼泽红假单胞菌菌体的SOD酶和CAT酶活性明显提高,分别比普通菌高158.43%和54.51%。In order to explore the effect of selenium enrichment on nutritional compositions of Rhodopseudomonas palustris, general nutrients, amino acids, vitamin B family, nicotinic acid, β-caroteneare, superoxide dismutase （SOD） and catalase （CAT） in selenium-enriched Rhodopseudomonas palustris （sRP） and normal Rhodopseudomonas palustris （nRP） were compared. Results indicated that the contents of protein, soluble sugar and crude fat of sRP were higher than those of nRP, which exhibited an enhancement by 9.10%, 5.13% and 42.86%, respectively. Whereas, the ash content of sRP was lower than that of nRP with a decrease by 23.73%. In addition, totally 17 amino acids were detected in sRP, while only 16 amino acids was detected in nRP. Moreover, the contents of amino acids in sRP were much higher than those in nRP, especially for methionine and lysine with an increase by 100% and 9.2%, respectively. Vitamin B2, vitamin B6, nicotinic acid and carotenoid in sRP were 6.45%, 5.77%, 7.20% and 45.08%, which were also higher than those in nRP, respectively. However, the content of vitamin B1 in sRP was lower than nRP. The activities of SOD and CAT in sRP exhibited an enhancement of 158.43% and 54.51% when compared with those in nRP. Therefore, selenium-enrichment can improve nutritional quality of Rhodopseudomonas palustris.

关 键 词：生物富硒 沼泽红假单胞菌 营养成分 评价 

分 类 号：TS202.3[轻工技术与工程—食品科学] Q939.112[轻工技术与工程—食品科学与工程]