产抗氧化胞外多糖海洋细菌的筛选及发酵产糖培养基优化  

Screening and Optimization of Fermentation Medium of an Antioxidant Exopolysaccharide-producing Marine Strain

作　　者：房耀维[1] 吕明生[1] 徐炜枫[2] 刘姝[1] 焦豫良[1] 王淑军[1] 

机构地区：[1]淮海工学院海洋学院 江苏省海洋资源研究际,江苏连云港222005 [2]江苏省农产品质量检验测试中心,江苏南京210036

出　　处：《食品科学》2010年第23期276-280,共5页Food Science

基　　金：江苏省高校自然科学基础研究面上项目(08KJB550001)

摘　　要：从连云港海域分离海洋细菌,并从中筛选获得一株产抗氧化活性较强的胞外多糖的细菌菌株OST23a。通过形态学、生理生化鉴定及16SrRNA分析,将菌株OST23a鉴定为Bacillussubtilis。采用单因素试验确定菌株OST23a发酵产胞外多糖的最佳碳、氮源和金属离子分别为正己烷、酵母提取物和CaCl2。采用正交试验确定了菌株OST23a产胞外多糖培养基的配方为:正己烷1.5%、酵母提取物0.1%、K2HPO40.3%、KH2PO40.1%、CaCl20.1%。菌株在该培养基产胞外多糖可达到9.06mg/L。In this study, a marine strain named OST23a with the ability to produce antioxidant exopolysaccharide （EPS） was screened out of the strains isolated from the samples of sea water or sea mud collected from the sea area near Lianyungang. The strain OST23a was identified as Bacillus subtilis according to its morphological, physiological, biochemical characteristics and 16S rRNA sequence. The EPS produced by this strain exhibited an obvious antioxidant activity. The optimal carbon and nitrogen sources as well as metal ion for the production of EPS were determined to be n-hexane, yeast extract and CaCl2 by single-factor experiments. The optimal formula of fermentation medium for the production of EPS by OST23a were explored to be 1.5% n-hexane, 0.1% yeast extract, 0.3% K2HPO4, 0.1% KH2PO4, and 0.1% CaCl2 by orthogonal array experiments. The productivity of EPS was up to 9.06 mg/L using this optimal medium.

关 键 词：海洋细菌 胞外多糖 抗氧化活性 筛选 培养基优化 

分 类 号：TS202.21[轻工技术与工程—食品科学]