瑶山甜茶可溶性糖的测定  

Determination of the soluble sugar in Rubus Suavissimus S.Lee

作　　者：邹青松[1] 陈山[1] 王晓[1] 陈玮[1] 李素霞[1] 韦艳君[1] 

机构地区：[1]广西大学糖业工程技术研究中心,广西南宁530004

出　　处：《食品工业科技》2011年第1期296-299,共4页Science and Technology of Food Industry

基　　金：广西区自然科学基金项目(0731051);广西区研究生创新项目(105930903060)

摘　　要：为探明瑶山甜茶中可溶性糖的含量和种类,采用蒽酮—硫酸比色法测定其可溶性总糖含量,运用高效凝胶过滤色谱法(HPGFC)测定多糖分子量分布范围,并利用高效液相色谱—蒸发光散射检测器(HPLC-ELSD)法在两种流动相体系中测定和分析其低分子寡糖和单糖的种类与含量。结果表明,瑶山甜茶中可溶性总糖含量为15.20%,相对标准偏差(RSD)为0.996%;多糖分子量Mw范围为51551～55628,RSD为0.64%～1.23%;单糖种类主要为木糖、果糖和葡萄糖,相对含量分别是0.54%、52.18%和42.76%。To investigate the content and component of the soluble sugar in R.Suavissirnus S.Lee, an anthronesulfuric acid chromatometry method was used to determine total soluble sugar content,and a high performance filter chromatography （HPGFC）was used to determine the molecular of its polysaccharide, and so was high performance liquid chromatography-evaporative light scattering detection （HPLC-ELSD）to determine the component and quantity of the monosaccharide and low-molecular-oligosaccharide.The results showed that the total soluble sugar was 15.2% with RSD of 0.996% ,the molecular range was between 51551-55628.Xylose,fructose and glucose was the main monosccharide in R.Suavissimus with a content of 0.54%, 52.18% and 42.76% respectively in area normalization method.

关 键 词：瑶山甜茶 可溶性糖 蒽酮-硫酸法 高效凝胶过滤色谱法(HPGFC) 高效液相-蒸发光散射法(HPLC-ELSD) 

分 类 号：TS207.2[轻工技术与工程—食品科学]