微生物发酵液中ε-聚赖氨酸的分离提纯  

Ion Exchange Resin Separation and Purification of ε-Poly-L-lysine from the Fermentation Broth of Streptomyces violaceusniger

作　　者：宗红[1] 詹耀[1] 吴翔[1] 阿茹罕[1] 冯凤琴[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310029

出　　处：《食品科学》2011年第1期131-134,共4页Food Science

摘　　要：筛选适用于分离提纯微生物发酵液中ε-聚赖氨酸的树脂并确定其工艺条件。以ε-聚赖氨酸吸附量和回收率为考察指标,采用静态吸附法选择适合的树脂,采用动态吸附法确定提取工艺。结果表明,弱酸型阳离子交换树脂HD-2对ε-聚赖氨酸具有良好的吸附分离性能。其动态分离提纯工艺条件为:层析柱为22mm×300mm时,上样流速为3mL/min;以0.1mol/L的HCl为洗脱液,洗脱流速为3mL/min。此工艺条件下,ε-聚赖氨酸的回收率为92.0%。The high-yieldε-poly-L-lysine-producing Streptomyces violaceusniger strain isolated and preserved in our laboratory was cultured to produceε-poly-L-lysine in this study.The mycelia-free supernatant obtained after the centrifugal separation of the fermentation broth was ultrafiltrated through 5 kD（larger than the molecular weight of ε-poly-L-lysine） polysulfone membrane and the filtrate was collected.The static adsorption and desorption characteristics of the filtrate on three different types of ion exchange resins were compared,and the dynamic adsorption and desorption of the filtrate on the selected resin were studied with respect to the effect of operation conditions on adsorption quantity and recovery.Based on the results from static adsorption and desorption,weakly acidic cation exchange resin HD-2 had excellent separation performance onε-poly-L-lysine.The best purification process for ε-poly-L-lysine was that the sample to be separated was applied to a 22 mm×300 mm HD-2 column with a flow rate of 3 mL/min,followed by 0.1 mol/L NaCl elution with the same flow rate.Aε-poly-L-lysine recovery of 92.0% was achieved using the optimized purification process.

关 键 词：Ε-聚赖氨酸 发酵液 分离提纯 阳离子交换树脂 

分 类 号：TS201.3[轻工技术与工程—食品科学]