地衣芽孢杆菌A产碱性蛋白酶的研究  

Study on Alkaline Protease from Bacillus licheniformis A

作　　者：张文杰[1] 高翔[1] 孙静[1] 姜李雁[1] 丁进锋[1] 岳福鹏[1] 苏秀榕[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,浙江宁波315211

出　　处：《食品科学》2011年第1期199-202,共4页Food Science

基　　金：浙江省重大科技攻关项目(2009C03017-1)

摘　　要：研究从经过60Co照射的东海香参水解液中分离出的地衣芽孢杆菌A产碱性蛋白酶的产率、分离纯化与生化特性。经硫酸铵沉淀、DEAE-Sepharose离子交换层析和Sephadex G-75凝胶层析分离纯化后,碱性蛋白酶产率为5.8%;经SDS-PAGE电泳测得其分子质量为35kD。酶的最适反应温度为50℃,最适pH值为11,热稳定性较好。该蛋白酶具有一定的耐氧化能力。The alkaline protease produced by the fermentation of Bacillus licheniformis A,a strain isolated from the hydrolysate of 60Co-irradiated Acaudina leucoprocta was salted out from the fermentation supernatant with ammonium sulfate.After DEAE-Sepharose ion exchange chromatographic separation and subsequent Sephadex G-75 gel permeation chromatographic purification,an alkaline protease yield of 5.8% was achieved.The alkaline protease was determined by SDS-PAGE to have a molecular weight of 35 kD.The optimal reaction temperature and pH of the enzyme were 50 ℃ and 11,respectively.The enzyme had perfect thermostability and a certain level of resistance to oxidation.

关 键 词：地衣芽孢杆菌A 碱性蛋白酶 分离纯化 

分 类 号：Q814.1[生物学—生物工程]