滑子蘑菌渣粗纤维素酶浸提及其酶学特性研究  

Extraction of Crude Cellulase from Pholiota nameko Residue and Studies on Enzymological Properties of Crude Cellulase

作　　者：马怀良[1] 龚振杰[1] 任荣[1] 李大伟[1] 郭文学 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157012 [2]牡丹江市绿珠果蔬技术开发有限责任公司,黑龙江牡丹江157000

出　　处：《中国食用菌》2011年第1期48-50,共3页Edible Fungi of China

基　　金：国家科技部科技人员服务企业行动资助项目(2009GJB20022);国家"十一五"科技支撑计划(2007BAD89B05-13)

摘　　要：利用水浸提法浸提滑子蘑菌渣纤维素酶,选取浸提时间、液料比和温度做单因素试验,然后通过正交试验优化滑子蘑菌渣纤维素酶的浸提工艺,并对结果进行了统计分析。结果表明,浸提时间、液料比和温度对纤维素酶浸提均有极显著性影响,最佳浸提条件为温度30℃、时间1.5 h、液料比50∶1。滑子蘑菌渣羧甲基纤维素酶活力最适pH为4.4,最适温度为70℃;滤纸酶活力最适pH为6.0,最适温度为60℃。In this study,water extract method was adopted to extract crude cellulase from Pholiota nameko residue.While liquid-material ratio and temperature were selected as single factor experiment,and then orthogonal experiments of extracting cellulase were conducted and its results were statistically analyzed.Results indicated that extraction time,extraction liquid-material ratio and extraction temperature exhibited a highly significant effect on crude cellulase from Pholiota nameko residue.It was the optimal condition that extraction temperature was 30℃,extraction time was 1.5 h and extraction liquid-material ratio was 50∶1.The optimum pH and temperature of CMCase activity were 4.4 and 70℃,and that of FPA（Filter Paper Activity） were 6.0 and 60℃.

关 键 词：纤维素酶 浸提工艺 菌渣 滑子蘑 酶学特性 

分 类 号：S646.16[农业科学—蔬菜学]