液相色谱/元素分析-同位素比值质谱联用法鉴定蜂蜜掺假  

Honey adulteration detection using liquid chromatography/elemental analysis-isotope ratio mass spectrometry

作　　者：费晓庆[1] 吴斌[1] 沈崇钰[1] 丁涛[1] 李丽花[1] 路颖[1] 

机构地区：[1]江苏出入境检验检疫局食品实验室,江苏南京210001

出　　处：《色谱》2011年第1期15-19,共5页Chinese Journal of Chromatography

基　　金：国家质量监督检验总局"食品中同位素溯源特征物质与掺假研究"课题

摘　　要：采用液相色谱/元素分析-同位素比值质谱联用法(LC/EA-IRMS)对国内蜂蜜掺假情况进行了研究。基于测定得到的38个纯正蜂蜜样品的碳同位素δ13C值数据,提出了纯正蜂蜜样品的δ13C值要求:蛋白质和蜂蜜的δ13C差值(Δδ13CP-H)≥-0.95‰,果糖和葡萄糖的δ13C差值(Δδ13CF-G)在-0.64‰至0.53‰范围内,各个组分间的δ13C最大差值(Δδ13Cmax)<2.09‰。对150个日常检测样品、蜂农和蜂蜜供应商的蜂蜜样品分别采用本文建立的LC/EA-IRMS和国家标准方法(EA-IRMS)进行鉴定,LC/EA-IRMS方法检出58个掺有C3或C4植物糖浆的阳性样品,而EA-IRMS方法仅检出7个掺有C4植物糖浆的阳性样品,可见新方法大大提高了对蜂蜜掺假的鉴别能力。A new method for honey adulteration detection using liquid chromatography/elemental analysis-isotope ratio mass spectrometry（LC/EA-IRMS） was developed.Based on the individual δ^13Cvalues detected for 38 authentic honey samples,the limits for the authentic honey samples were proposed： the δ^13Cdifference between protein and honey（Δδ^13C-H） should be higher or equal to than-0.95‰,the δ^13Cdifference between fructose and glucose（Δδ^13C-G） should be from-0.64‰ to 0.53‰,and the maximum difference of δ^13Cvalues between all the components（Δδ^13Cax） should be lower than 2.09‰.Based on the above criteria,the 58 positive samples spiked with C4 or C3 plant sugar syrup were confirmed by LC/EA-IRMS method from 150 commercial honey samples,while only 7 samples spiked with C4 plant sugar syrup were confirmed by the official EA-IRMS method.The proposed method represents a significant improvement in comparing with the official EA-IRMS method.

关 键 词：液相色谱 元素分析 同位素比值质谱 掺假 蜂蜜 

分 类 号：O658[理学—分析化学]