黄参粗多糖脱蛋白工艺以及对亚硝酸盐清除作用的研究  

Study on removal process of proteins from crude Sphallerocarpus gracilis polysaccharides and its scavenging capability to nitrite

作　　者：郭小鹏[1] 田呈瑞[1] 高春燕[1] 孟友武 

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710062 [2]甘肃省山丹军马场食品公司,甘肃张掖734104

出　　处：《食品工业科技》2011年第2期274-276,共3页Science and Technology of Food Industry

摘　　要：采用Sevag法、TCA-Sevag法、木瓜蛋白酶-Sevag法对黄参粗多糖脱蛋白工艺进行了研究。另外,研究了体外模拟胃液条件下黄参多糖对亚硝酸盐的清除作用,采用分光光度法测定了黄参粗多糖对亚硝酸盐的清除率。实验结果表明,通过比较三种脱蛋白方法,木瓜蛋白酶-Sevag法较好,累计蛋白去除率可达68.65%,累计多糖损失率17.14%。黄参多糖对亚硝酸盐清除最佳方法为:向25mL模拟胃液中加入50mg黄参多糖,反应时间为15min,对亚硝酸盐的清除率最高,可达86.6%。Sevag method,TCA-Sevag and Papain-Sevag method of removing protein process from crude S. gracilis polysaccharides were studied. In addition,under the condition of simulated gastric juice in vitro,the effect of S. gracilis polysaccharide to scavenge nitrite was studied,using spectrophotometric to determine the parameters of the S.gracilis polysaccharide on clearance rate of nitrite. Experimental results showed that the method of removal of protein by comparing the three kinds of excellent,Papain-Sevag method was better,the accumulated protein in the removal rate was up to 68.65%,the cumulative loss rate of polysaccharide was 17.14%. S.gracilis polysaccharide on the best method for scavenging nitrite：adding 50mg S.gracilis polysaccharide to the 25mL simulated gastric juice,reaction time was 15min,the scavenging rate reached the maximum 86.6%.

关 键 词：黄参 脱蛋白 亚硝酸盐 清除 

分 类 号：TS201.23[轻工技术与工程—食品科学]