共轭亚油酸两种主要异构体冷冻结晶分离工艺研究  

Study on separation of conjugated linoleic acid two main isomers by freezing crystallization

作　　者：衣丹[1] 林学政[1] 刘发义[1] 梁晓芳 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]青岛澳海生物有限公司,山东青岛266101

出　　处：《食品与机械》2011年第1期26-28,共3页Food and Machinery

基　　金：国家高技术研究发展计划"863"资助课题(编号:2007AA091905)

摘　　要：应用冷冻结晶技术,对共轭亚油酸两种主要异构体c9,t11 CLA和t10,c12 CLA进行分离。结果表明,通过尿素包合法,去除原料中的饱和脂肪酸,然后在乙醇作溶剂情况下,将乙醇与共轭亚油酸乙酯按18:1配比,置于-56℃低温冷柜中,反应96 h后得沉淀结晶产品,其所含的t10,c12 CLA比c9,t11 CLA高达12.28:1,共轭亚油酸总含量为95.17%。The conjugated linoleic acid two main isomers were separated by freezing crystallization.At first the saturated fatty acid was removed by urea adduction fractionation,then the conjugated linoleic acid was frozen at-56 ℃ in ethanol,to improve the ratio of two isomers.At the optimal conditions： ethanol dosage 18∶1（based on CLA）,after reaction 96 h,t10,c12CLA： c9,t11 CLA=12.28∶1,and the production of CLA contented in ethyl ester was up to 95.17%.

关 键 词：共轭亚油酸 异构体 冷冻结晶 

分 类 号：TS201.2[轻工技术与工程—食品科学]