影响农残快检仪抑制率测定结果的因素  

Factors Influencing the Determination of Enzymatic Inhibition Rate of Pesticide Residues on A Rapid Analyzer

作　　者：杜美红[1] 孙永军[1] 陈舜琮[1] 

机构地区：[1]北京市理化分析测试中心,北京100089

出　　处：《食品科学》2011年第4期200-202,共3页Food Science

基　　金：科技部基础性工作专项(2008IM0401400)

摘　　要：考察不同因素对农残快检仪抑制率的影响。以GDYN-1096SC型农药残留快检仪为例,考察快检仪的重复性、稳定性、吸光度线性、通道一致性4个技术参数,并使用乙酰胆碱酯酶和小麦酯酶两种来源不同的酶,分别对甲胺磷、对硫磷、敌敌畏、氧化乐果、毒死蜱、甲萘威、灭多威、克百威8种农药进行检测。结果显示:农残快检仪各技术参数在满足测试要求的情况下,不影响抑制率;来源不同的酶试剂是影响快检仪抑制率测定结果的主要因素。A wide variety of analytical instruments have been recently developed for the rapid determination of pesticides using the principle of enzymatic inhibition. In this study,a GDYN-1096SC type rapid pesticide residue analyzer was selected to determine 8 kinds of pesticides such as methamidophos,prarathion,dichlorvos,omethoate,chlorpyrifos,carbaryl,methomyl and carbofuran with acetylcholinesteras or wheat esterase,two enzymes from different sources based on measurements of repeatability,stability,absorbance linearity and channel consistency of the instrument. When various technical parameters met the requirements for testing,the analyzer could accurately determine pesticides. Enzymes from different sources,however,were the main factors that influence the determination of enzymatic inhibition rate of pesticide.

关 键 词：酶抑制法 农药残留 农残快检仪 乙酰胆碱酯酶 小麦酯酶 

分 类 号：TS207.5[轻工技术与工程—食品科学]