稻草液化产物纤维素的酶解糖化研究  

Enzymatic-hydrolysis of liquefied product from rice straw

作　　者：刘德龙[1] 张玉苍[1] 何连芳[1] 孙岩峰[1] 杨红[1] 丛日昕[1] 

机构地区：[1]大连工业大学资源开发利用研究所,辽宁大连116034

出　　处：《中国酿造》2011年第2期55-58,共4页China Brewing

基　　金：辽宁省高校优秀人才支持计划项目(2008RC08);辽宁省工业攻关计划项目(2009223003)资助

摘　　要：研究了稻草经乙二醇"液化"后的酶解糖化条件,得到了可用于生产2,3-丁二醇等燃料的还原糖。结果表明,经"液化"预处理,纤维素含量由36.7%上升为61.2%,酶解所需用酶量、时间远远少于未处理的秸秆;150g/L的"液化"产物,加酶量68.8IU/g,pH4.8,温度50℃,转速200r/min,酶解108h,得到葡萄糖75.5mg/mL,总还原糖得率为51.5%。用得到的酶解液适量补糖发酵生产2,3-丁二醇,最终得到2,3-丁二醇29.4mg/mL,摩尔转化率达到理论值的73.5%。In order to obtain the reducing sugar to produce 2,3-butanediol, the optimal enzymatic-hydrolysis conditions of rice straw liquefied by ethylene glycol was investigated in this paper. The results showed that the content of cellulose was rise to 61.2% from 36.7% after pretreatment, and the amount of enzyme and the time of enzymatic-hydrolysis were decreased. The optimal condition of enzymatic hydrolysis were as followed： substrate 150g/L, the amount of enzyme 68.91U/g, pH value 4.8, temperature 50℃, rotation speed 150r/rain and the hydrolysis time 108h. Under these conditions, the yield of glucose and reducing sugar hydrolysis were 75.Smg/ml and 51.5%, respectively. With fed-batch of glucose, the yield of the 2, 3-butanediol and the molar ratio of 2, 3-butanediol were 29.4mg/ml and 73.5%, respectively.

关 键 词：稻草 液化 酶解 2 3-丁二醇 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]