金属离子对木聚糖酶制备阿魏酸糖酯的影响  

Influence of Ions on Feruloyl Oligosaccharide Preparation from Wheat Bran by Xylanase Hydrolysis

作　　者：赵冰[1,2] 刘海顺 王静[1,2] 张可[4] 孙宝国[1,2] 曹雁平[1,2] 

机构地区：[1]北京工商大学北京市植物资源研究开发重点实验室,北京100048 [2]食品添加剂与配料北京高校工程研究中心,北京100048 [3]中储粮北京分公司承德粮油质检中心,河北省承德市双滦区东平台,河北承德067001 [4]空军航空医学研究所,北京100142

出　　处：《食品科学》2011年第3期19-22,共4页Food Science

基　　金：北京市教育委员会面上项目(KM200910011002);北京市科技新星项目(2008B07)

摘　　要：采用木聚糖酶制备小麦麸皮中的阿魏酸糖酯。结果表明:添加金属离子会明显影响木聚糖酶的活性,K+、Ca2+、Mn2+、Ag+、Zn2+、Co2+对木聚糖酶有激活作用,其中Mn2+、Ag+、Zn2+、Co2+有较强的激活作用;而EDTA、Sn2+、Fe2+、Fe3+、Hg2+、Mg2+对木聚糖酶有抑制作用,而且高浓度的金属离子也会抑制木聚糖酶的活性。在制备阿魏酸糖酯时添加一定浓度对木聚糖酶有激活作用的金属离子,可以降低木聚糖酶的添加量。The feruloyl oligosaccharide was prepared from wheat bran by xylanase. The results indicated that the activity of xylanase was strongly affected by metal ions. K^＋, Ca^2＋, Mn2＋, Ag^＋, Zn^2＋, and Co^2＋ could activate xylanase, and Mn^2＋, Ag^＋, Zn^2＋ and Co^2＋ showed stronger promoting effect. The activating effect of Zn^2＋ was positively dependent on its concentration within a certain range, but excessively high concentration inhibited the activity of the enzyme. However, EDTA, Sn^2＋, Fe^2＋, Fe^3＋, Hg^2＋ and Mg^2＋ were inhibitors of the enzyme. Less xylanase was required when adding a certain concentration of metal ions during preparation of feruloyl oligosaccharide.

关 键 词：麦麸 金属离子 木聚糖酶 阿魏酸糖酯 

分 类 号：Q814[生物学—生物工程]