Lachnum YM328多糖发酵条件优化及抗氧化性  

Optimization of fermentation conditions of extracellular polysaccharide by Lachnum YM328 and its antioxidant activity

作　　者：陈吴西[1] 蔡敬民[2] 邱涛[1] 张利兵[1] 叶明[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]合肥学院生物与环境工程系,安徽合肥230022

出　　处：《合肥工业大学学报（自然科学版）》2011年第2期300-303,312,共5页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31070021);安徽高等学校省级自然科学研究重大资助项目(2009kj05zd)

摘　　要：文章在碳源、氮源、金属离子和发酵时间4个单因素试验的基础上,利用正交试验法对Lachnum YM328胞外多糖发酵条件进行了研究,得出最优发酵条件为:ρ(葡萄糖)=20 g/L,ρ(酵母膏)=5 g/L,ρ(硫酸锰)=0.075 g/L,25℃下培养10 d;该条件下YM328胞外多糖产量为1.895 mg/mL,相对于优化前提高了36.27%;通过测定其胞外多糖(EPS)对.OH、NO2-的清除作用来研究YM328胞外多糖的抗氧化性;结果表明,YM328胞外多糖对.OH、NO2-均具有明显的清除作用,抗氧化性随着多糖质量浓度的增加呈增强趋势。Based on single-factor tests（carbon source,nitrogen source,metal ions and culture time）,the optimized fermentation conditions of Lachnum YM328 extracellular polysaccharide were determined by orthogonal methodology as follows： glucose 20 g/L,yeast extract 5 g/L,manganese sulfate monohydrate 0.075 g/L,at 25 ℃ culturing for ten days.Under these conditions,the extracellular polysaccharide yield reached 1.895 mg/mL,and increased by 36.27% in comparison with the un-optimized condition.The antioxidant activity of YM328 extracellular polysaccharide was studied by measuring the scavenging capacity of the ·OH and nitroso radical.The results show that the YM328 extracellular polysaccharide plays a significant role in reducing the ·OH and nitroso radical,and the antioxidant activities are elevated by the concentration increase of extracellular polysaccharide.

关 键 词：多糖 发酵条件 羟基自由基 亚硝基自由基 粒毛盘菌 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]