胶原蛋白肽对高脂日粮小鼠抗氧化能力和血脂代谢的影响  

Effects of Collagen Peptides on Antioxidant Capacity and Serum Lipid Metabolism of Mice Fed with High-fat Diet

作　　者：李亚欣[1] 施用晖[2,1] 王洁昀[1] 林云鉴[1] 乐国伟[2,1] 

机构地区：[1]江南大学食品学院,无锡214122 [2]江南大学食品科学与技术国家重点实验室

出　　处：《天然产物研究与开发》2011年第1期54-58,68,共6页Natural Product Research and Development

基　　金：十一五"863项目":蛋白质的定向酶解及生物活性肽富集纯化研究(2007AA0Z325);国家科技支撑计划课题:抗氧化功能食品的研究与产业化(2006BAD27B06)

摘　　要：本文探讨胶原蛋白肽对高脂日粮小鼠抗氧化能力及血脂代谢的影响。40只小鼠随机分为5组(n=8):分别饲喂正常日粮,高脂日粮,添加0.5%和1%胶原蛋白肽及0.1%硫辛酸的高脂日粮。42 d后测定小鼠全血(血清)和组织活性氧(Reactive oxygen species,ROS)水平、丙二醛(Malondialdehyde,MDA)含量、抗氧化酶活力及血脂水平。结果表明:0.5%和1%胶原蛋白肽都可显著降低机体ROS水平及MDA含量(P<0.05),增强抗氧化能力(P<0.05),改善血脂水平(P<0.05)。1%胶原蛋白肽能使机体抗氧化能力及血脂水平恢复到正常水平,0.5%的添加量效果不显著。适量的胶原蛋白肽可有效提高机体抗氧化能力,缓解高脂膳食造成的氧化应激,改善血脂代谢。The effects of collagen peptides on antioxidant capacity and serum lipid metabolism of mice fed with high-fat diet were studied in the present work. Forty mice were randomly divided into 5 groups. They were fed with ordinary diet, high-fat diet,and high-fat diet with 0. 5% collagen peptides,1% collagen peptides,and 0. 1% hpoic acid,respectively. The level of reactive oxygen species （ROS） ,contents of malondialdehyde （MDA） and antioxidant enzymes activities in blood （serum） and tissues and serum lipid level were determined after 42 days. The results showed that both 0.5% and 1% collagen peptides could remarkably decrease the levels of ROS and the contents of MDA, enhance the antioxidant capacity of mice, as well as improve the level of serum lipid. The antioxidant capacity and serum lipid level could recover to normal level when 1% collagen peptides was added to high-fat diet. However,there were no significant effects in O. 5% collagen pepfides group. These results indicated that the appropriate collagen peptides could effectively enhance antioxidant capacity, relieve oxidative stress caused by high-fat diet and improve serum lipid metabolism.

关 键 词：胶原蛋白肽 硫辛酸 高脂日粮 氧化应激 抗氧化能力 血脂代谢 

分 类 号：R151.2[医药卫生—营养与食品卫生学] R285[医药卫生—公共卫生与预防医学]