Acidothermus cellulolyticus 11B葡萄糖异构酶基因的克隆、表达及酶活性研究  

Cloning,expression and characterization of Acidothermus cellulolyticus 11B glucose isomerase

作　　者：王晓乐[1] 薛庆海[1] 江波[1] 沐万孟[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品工业科技》2011年第3期192-194,共3页Science and Technology of Food Industry

基　　金："十一五"国家863资助项目(2006AA10Z334)资助

摘　　要：葡萄糖异构酶是生物法转化葡萄糖为果糖制备果葡糖浆的关键酶。本文克隆到一种葡萄糖异构酶基因xyl,来源于嗜酸耐热型微生物Acidothermus cellulolyticus 11B(ATCC43068)。以pET-22b(+)为载体质粒,E.coliBL21(DE3)为宿主细胞,构建了基因重组菌,IPTG可诱导目的重组葡萄糖异构酶过量表达;经亲和层析纯化的重组蛋白样品进行SDS-PAGE分析,在约43kDa处出现显著的特征蛋白条带;活性检测结果表明该重组葡萄糖异构酶具有较高的果糖转化活性。Glucose isomerase is the main enzyme for biotransformation of high fructose corn syrup. A gene,encoding glucose isomerase from Acidothermus cellulolyticus 11B（ATCC 43068） was cloned and over expressed in Escherichia coli. The recombinant glucose isomerase was purified to electrophoretic homogeneity by affinity chromatography. The recombinant enzyme molecular weight was about 43kDa with high bioconversion activity.

关 键 词：葡萄糖异构酶 果葡糖浆 克隆 表达 亲和层析 

分 类 号：Q789[生物学—分子生物学]