食品中单核细胞增生性李斯特菌的快速分离鉴定  

Rapid isolation and identification of Listeria monocytogenes in foods

作　　者：何勇琴[1] 潘迎捷[1] 卢瑛[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《食品工业科技》2011年第3期215-218,共4页Science and Technology of Food Industry

基　　金：上海海洋大学博士启动基金(B-8203-08-0220);上海市教委创新项目(09YZ274);国家科技支撑计划项目(2008BAD94B09)

摘　　要：单核细胞增生性李斯特菌(Listeria monocytogenes,简称单增李斯特菌)是一种重要的食源性致病菌,能引起人畜共患李斯杆菌病。本文采用两种不同的增菌分离方法从散装牛奶和猪肉中共分离得到8株单增李斯特菌。通过革兰氏染色、生化鉴定、PCR扩增hlyA基因、16SrDNA测序、血清分型等一系列实验对可疑菌株进行分析鉴定。综合实验结果和其他单增李斯特菌分离标准,探讨开发了一种快速分离鉴定单增李斯特菌的方法,该方法可在4～5d内完成单增李斯特菌的分离检测过程。Listeria monocytogenes is an important food borne pathogenic which can cause listeriosis both in human and animals. 8 Listeria monocytogenes were isolated from bulk milk and pork using two different enrichment isolation methods. A series of experiments（Gram stain,biochemical identification,PCR amplification of hlyA gene,16S rDNA sequencing and serotyping） were employed to analyze and identify the suspicious colonies. A rapid method to isolate and identify Listeria monocytogenes was developed on the basic of the experimental results and the other separation standards. The detection process can be completed within 4 ~ 5d using the above method.

关 键 词：单增李斯特菌 快速 分离 鉴定 hlyA基因 血清分型 

分 类 号：TS201.1[轻工技术与工程—食品科学]