陕西部分地区不同食源性大肠杆菌耐药性检测  

Drug Resistance Detection of Escherichia Coli from Different Food Origins in Some Districts of Shaanxi Province

作　　者：只帅[1] 席美丽[1] 刘攻关[1] 吴淑鹏[1] 殷童[1] 姚远[1] 杨保伟[1] 孟江洪[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《中国食品学报》2011年第1期196-201,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：后稷学者基金项目;回国人员资助项目

摘　　要：对陕西西安市和杨凌示范区超级市场及农贸市场零售肉及凉拌菜中的大肠杆菌耐药性及肠产志贺样毒素大肠杆菌进行检测。采用美国临床实验室标准化委员会(National Committee of Clinical Laboratory Standard,NCCLS)推荐的琼脂稀释法,以大肠埃希氏菌ATCC25922、金黄色葡萄球菌ATCC25923及粪肠球菌ATCC29212为质控菌株,对来源于不同食品的748株大肠杆菌进行15种抗生素药敏性检测。发现大肠杆菌分离株对链霉素的抗性最高(88.2%),其次为四环素(84.4%)、甲氧苄啶-新诺明(64.4%)、萘啶酮酸(62.6%)、氨苄西林(56.0%)、环丙沙星(39.2%)、氯霉素(37.6%)、卡那霉素(34.9%)、庆大霉素(34.2%)、阿莫西林-克拉维酸(28.9%)、加替沙星(26.9%)。所有受试菌株对阿米卡星的敏感性最高(8.3%),其次为头孢西丁(11.5%)、头孢曲松(13.8%)、头孢哌酮(19.5%)。多重耐药大肠杆菌分离率为73.9%。从猪肉及羊肉源样品中分离到肠产志贺样毒素大肠杆菌2株。陕西西安地区食品中大肠杆菌耐药性比较严重。Antibiotic resistance and prevalence of Shiga Toxin-producing Escherichia coli were determined among Escherichia coli isolates collected from retail meats and ready-to-eat food in supermarkets and open markets in Xi＇an and Yangling areas of Shaanxi Province.Antimicrobial Susceptibility to 15 antibiotics of 748 Escherichia coli isolates were determined by using agar dilution methods,which was recommended by National Committee of Clinical Laboratory Standard（NCCLS）,and using E.coli.ATCC25922,E.faecalis ATCC29212,Staphylococcus Aureus ATCC25923 as quality control strains.The results indicated that the isolates were most resistant to streptomycin at 88.2%,followed by resistance to tetracycline（84.4%）,trimethoprim-sulfamethoxazole（64.4%）,nalidixic acid（62.6%）,ampicillin（56.0%）,ciprofloxacin（39.2%）,chloramphenicol（37.6%）,kanamycin（34.9%）,gentamicin（34.2%）,amoxicillin-clavulanic acid（28.9%）,gatifloxacin（26.9%）.The isolates displayed lower resistance to amikacin（8.3%）,cefoxitin（11.5%）,ceftriaxone（13.8%）,cefoperazone（19.5%）.73.9% of the isolates were multidrug-resistant.Two Shiga Toxin-producing Escherichia coli isolates were isolated from pork and mutton samples.Escherichia coli isolates recovered from retail meats and ready-to-eat food in Xi＇an district were seriously resistant to common antibiotics.

关 键 词：食品 大肠杆菌 多重耐药性 肠产志贺样毒素大肠杆菌 

分 类 号：R155.5[医药卫生—营养与食品卫生学]