酶法水解蚕蛹蛋白制备多肽的工艺研究  

Preparation of Peptide From Silkworm Pupa Protein With Enzymic Hydrolysis

作　　者：熊燕飞[1] 王惠平[1] 陈怀新[1] 杨艳燕[1] 

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》1999年第3期287-290,共4页Journal of Hubei University：Natural Science

基　　金：湖北省重点科技研究资助项目

摘　　要：研究了几种蛋白酶对桑蚕蛹蛋白粉的水解情况 ,采用均匀设计法对桑蚕蛹蛋白粉的限制水解条件进行了优化选择 ,得到胰酶水解桑蚕蛹蛋白粉的优化条件为 :酶用量占底物的质量分数为 4 50 % ,底物含量的质量体积分数为 1 5% ,反应温度为 4 0℃ ,水解时间为 6 0h ,水解pH值为 8 0 ,在此条件下水解 ,酸溶性肽得率可达 92 3 7% .An optimum technology of peptide preparation from silkworm pupa protein by means of hydrolysis with pancreatin was studied through uniform design.The optimum conditions for the limited hydrolysis of silkworm pupa protein were concluded as follows:the ratio of enzyme to substrate ( E/S )is 4.5%( W/W ),the substrate concentration ( S ) is 1.5% ( W/V ),the recreation occurs at 40℃ and pH 8.0 for 6 h.And the yield of peptide is 92 37 %.

关 键 词：酶 水解 蚕蛹蛋白 多肽 工艺 

分 类 号：TQ936.16[化学工程]