脂溶性茶多酚抗氧化特性的研究  

Antioxidant properties of liphophilic tea polyphenols

作　　者：梁燕[1] 沈生荣[1] 杨贤强[1] 赵保路[2] 侯京武[2] 

机构地区：[1]浙江大学茶学系,浙江杭州310029 [2]中科院生物物理研究所,北京100101

出　　处：《浙江大学学报（农业与生命科学版）》1999年第5期529-534,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省教委资助

摘　　要：从化学、仿生物、细胞三个层次，水溶、脂溶两种体系研究了脂溶性茶多酚（ＬＴＰ）的抗氧化特性，并与维生素Ｅ（ＶＥ ），绿茶多酚（ＧＴＰ）等进行了比较⒚结果表明：对水溶性体系中的·ＯＨ，巨噬细胞呼吸爆发产生的氧自由基的清除效果为ＧＴＰ＞ ＬＴＰ＞ ＶＥ⒚对大分子稳定的二苯基苦基肼基（ＤＰＰＨ）自由基，ＬＴＰ，ＧＴＰ效果接近且均显著优于ＶＥ⒚在膜体系中，ＧＴＰ对Ｆｅ２＋ 诱导的膜脂质过氧化的抑制作用优于ＬＴＰ，ＶＥ⒚而ＶＥ 对２，２＇偶氮２，４二甲基戊腈（ＡＭＶＮ）诱导的膜脂质过氧化的抑制作用显著优于ＬＴＰ， ＧＴＰ，当浓度大于５０ μｇ／ｍ Ｌ时，ＬＴＰ的抑制作用保持在２５％ 左右并显著优于ＧＴＰ⒚ＬＴＰ基本保留了原有茶多酚优良的抗氧化特性，在脂溶性体系中效果较好⒚同时给出了抗氧化剂在各体系中 ５０％抑制率或清除率时的浓度，即ＩＣ５０In order to investigate the antioxidant properties of the novel antioxidant lipophilic tea polyphenols (LTP),we established three levels of models from chemical, imitate biological and biological systems, and compared their activities with Vitamin E (V E) and green tea polyphenols (GTP).It was found that their ability to scavenge hydroxyl radicals (·OH), and reactive oxygen species generated by macrophage's breathe burst in biological system decreased in the order of GTP>LTP>V E. In big molecular stable free radical DPPH (1,1 diphenyl 2 picylhydrazyl) system, GTP and LTP have close scavenge activities and significantly better than that of V E. It was also found that they could inhibit lipid peroxidation of liposomal suspension induced by iron and was classified as follows: GTP>V E≈LTP. The inhibitary activity on the lipid peroxidation initiated by 2,2' azobis(2,4 dimethylvaleronitrile)(AMVN) is decreased in order of V E>LTP≈GTP,when the concentration is more than 50 μg/mL ,their antioxidant activities could be classified as follows: V E >LTP>GTP. The concentration of these compounds inhibiting 50% lipid peroxidation (IC 50 )or scavenging 50% free radicals(SC 50 ) were also given in the paper.

关 键 词：脂溶性茶多酚 维生素E 绿茶多酚 抗氧化特性 

分 类 号：S571.1[农业科学—茶叶生产加工] Q946.82[农业科学—作物学]