玉米醇溶蛋白提取工艺选择与优化  

Optimization and selection of extraction technology of corn zein

作　　者：相恒绪[1] 陈琦[2] 敬思群[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆维吾尔族自治区乌鲁木齐830046 [2]江苏科技大学生物与环境工程学院,江苏镇江212018

出　　处：《郑州轻工业学院学报（自然科学版）》2011年第1期34-38,共5页Journal of Zhengzhou University of Light Industry:Natural Science

基　　金：新疆维吾尔自治区科技支疆项目(200991236)

摘　　要：以玉米蛋白粉为材料,采用乙醇浸提法、碱提酸沉法和盐析法提取玉米醇溶蛋白,利用正交试验对提取条件进行了优化与选择.结果表明,乙醇浸提法最优,提取率高,变性程度最小.乙醇浸提法的最佳工艺条件为:提取温度60℃,料液比1 g/8 mL,浸提时间5 h,乙醇浓度80%,此时提取率可达82.21%,玉米醇溶蛋白变性率仅为10.23%.Corn zein was obtained via ethyl alcohol extraction method,alkali-extraction and acid precipitation method and salting-out method with corn gluten meal as raw material.Extraction conditions were optimized through orthogonal test.The results showed that ethyl alcohol extraction method was the optimal method which not only the extraction rate of zein was high,but also the denaturation degree of corn zein was weak.The optimum extraction technology of ethyl alcohol extraction method was obtained as follows：temperature 60 ℃,solid-liquid ratio 1 g/8 mL,time 5 h,ethanol concentration 80%;extraction rate was 82.21%,denaturation degree was 10.23%.

关 键 词：玉米醇溶蛋白 乙醇浸提法 碱提酸沉法 盐析法 

分 类 号：TS201.21[轻工技术与工程—食品科学]