茉莉花中芳樟醇生成相关酶的精制  

Purification of the enzyme concerned in linalool production from Jasminum Sambac Ait.

作　　者：董尚胜[1] 大石久泰[2] 坂田完三 童启庆[1] 渡边修治[2] 

机构地区：[1]浙江大学茶学系,浙江杭州310029 [2]静冈大学农学部

出　　处：《浙江大学学报（农业与生命科学版）》1999年第4期421-424,共4页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金

摘　　要：从酶学角度探讨了茉莉花重要香气成份芳樟醇的 生成机理⒚以双瓣茉莉花为材料，经过调制丙酮粉，缓冲液粗提，２０％ ～８０％ 饱和度硫铵分部， Ｃ Ｍ  Ｔｏｙｏｐｅａｒｌ ６５０ Ｍ 和 Ｆ Ｐ Ｌ Ｃ Ｃ Ｍ  Ｔｏｙｏｐｅａｒｌ ６５０ Ｓ两次柱层析，得到了 Ｆ Ｃ 蛋白分部液⒚同时经 Ｇ Ｃ Ｍ Ｓ 定性和 Ｇ Ｃ 定量分析，明确 Ｆ Ｃ 部分是催 化茉莉花粗 香气前 体物 质生 成芳 樟醇 的主 要部分⒚进 一步 经等 电电 泳、β Ｄ葡萄 糖苷 酶活 性染色 等电 电泳 和 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 凝胶电泳，初步判断 β Ｄ葡萄糖苷酶参与了芳樟醇的生成，且该酶的等电点在 ８．７～９．０，其单体蛋白的分子量在 ３５～３８ ｋ Ｄ 之间In this study, the mechanism of linalool production, which is a main constituent of Jasminum Sambac Ait. aroma, was probed in respect to enzymology. Fraction F C protein solution was obtained in bipetaloids Jasminum Sambac Ait. flowers through acetone powder preparation, buffer solution extraction, precipitation with 20% 80% saturated ammonium sulphate solution, CM Toyopearl 650 M and FPLC CM Toyopearl 650 S column chromatography successively, and was determined by GC MS qualitative analysis and GC quantification as the main catalytic promoter in the linalool production from its aroma precursors in Jasminum Sambac Ait.. Further identification with isoelectrophoresis, β glucosidase activity staining isoelectrophoresis, and SDS PAGE electrophoresis indicated that β glucosidase was involved in the linalool production, its isoelectric point appeared in 8.7 9.0,and that the molecular weight of its monomeric protein fell in 35 38 kD.

关 键 词：茉莉花 芳樟醇 酶 

分 类 号：TQ654.2[化学工程—精细化工]