沙雷氏菌Serratia sp.BK-98发酵生产2-酮基-D-葡萄糖酸的工艺优化及动力学研究  

Fermentation process optimization and kinetics studies of 2-keto-D-gluconic acid production by Serratia sp. BK-98

作　　者：张炜[1] 谢志鹏[1] 罗玮[2] 张建国[1] 

机构地区：[1]浙江大学生物化学研究所,浙江杭州310058 [2]浙江大学生物工程研究所,浙江杭州310027

出　　处：《化工学报》2011年第5期1371-1376,共6页CIESC Journal

摘　　要：引言 2-酮基-D-葡萄糖酸（2-KDG）有着广泛的用途,它能被用作食品添加剂、水泥增塑剂、洗涤剂,是照片显影剂的重要成分;同时它也是除草剂、D-核酮糖、D-阿拉伯糖,特别是D-异抗坏血酸合成过程中的重要前体。Based on the optimization of culture conditions for producing 2-keto-D-gluconic acid（2-KDG）by Serratia sp.BK-98 in a Erlenmeyer flask,the factors of dissolved oxygen（DO）and pH affecting 2-KDG batch fermentation in 100 L fermenter were further optimized to be 30% and 6.0 respectively.Under the DO-stat and pH-stat batch culture conditions,2-KDG production reached 211.2 g·L-1.The kinetics of DO-stat and pH-stat batch fermentation were also investigated and the models for biomass,substrate consumption and product were established respectively based on the Logistic equation,Leudeking-Piret equation and Modified Leudeking-Piret equation.Curve fittings for the above models by using experimental data were performed by the non-linear least squares method with the software Origin 8.0.With the evaluated model parameters,the calculated values of the models and experimental data were in good agreement and the models could provide guidance for 2-KDG fermentation production.

关 键 词：2-酮基-D-葡萄糖酸 发酵工艺 优化 动力学模型 SERRATIA sp.BK-98 

分 类 号：Q815[生物学—生物工程] TQ921.7[轻工技术与工程—发酵工程]