沙枣多酚超声波辅助提取工艺优化及抗氧化性研究  

Ultrasonic Assisted Extraction Technology Optimization and Its Antioxidant Activity of Elaeagnus Angustifolia L.Polyphenol

作　　者：王雅[1,2] 樊明涛[1] 赵萍[2] 任海伟[2] 董晓琳[2] 苏阿龙[2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]兰州理工大学生命科学与工程学院,兰州730050

出　　处：《中国食品学报》2011年第2期95-103,共9页Journal of Chinese Institute Of Food Science and Technology

摘　　要：采用响应曲面设计,研究超声波辅助提取时料液比、乙醇浓度、功率、超声时间及其交互作用对沙枣多酚提取效果的影响。用沙枣多酚对二苯代苦味酰自由基(DPPH·)的清除能力以及Rancimat法研究其抗氧化活性。用DesignExpert软件确定沙枣多酚超声波提取的最佳工艺参数是:超声波功率309.35W,时间9.71min,料液比1∶11.40,乙醇体积分数48.10%。沙枣多酚对DPPH·有显著的清除效果,且明显优于维生素C和单宁酸标准品。当质量浓度为3.5μg/mL时,沙枣多酚、维生素C和单宁酸标准品对DPPH·的最大清除率分别为97.30%、46.87%和34.17%。沙枣多酚对DPPH的清除效果与其浓度之间呈明显的量效关系。Rancimat法结果表明,沙枣多酚的抗氧化活性优于同浓度的维生素C和单宁酸标准品。The ultrasonic assisted extraction technology of Elaeagnus Angustifolia L.polyphenol was studied,and the response surface design was applied to analyze the influence of the raito of solid to liquid,concentration of ethanol,ultrasonic power and extraction time on the extraction results.The antioxidant activity of Elaeagnus Angustifolia L.polyphenol was also studied by useing clearance ability for DPPH· and Rancimat method.The result indicated that the optimum extracting conditions of Elaeagnus Angustifolia L.polyphenol were as follows：the ultrasonic power 309.35 W,the extraction time 9.71 min,the ratio of solid to liquid 1∶11.40 and concentration of ethanol 48.10%.Besides,scavenging activity of Elaeagnus Angustifolia L.polyphenol on DPPH· was higer than VC and tannic acid.When the concentration was 3.5 μg/mL of polyphenol,VC and tannic acid,the clearance rate of DPPH were 97.3%,46.87% and 34.17% respectively,and the scavenging activity was correlated with the concentration of Elaeagnus Angustifolia L.polyphenol.Result of Rancimat method showed that Elaeagnus Angustifolia L.polyphenol had stronger antioxidant activity compared with VC and tannic acid at the same concentration.

关 键 词：沙枣 多酚 超声波辅助提取 响应曲面法 抗氧化活性 

分 类 号：TQ461[化学工程—制药化工]