陕西省部分地区零售肉中沙门氏菌和奇异变形杆菌Ⅰ类整合子检测与耐药分析  

Class Ⅰ Integron and Antibiotic Resistance of Salmonella and Proteus mirabilis in Retail Meat from Shaanxi Province

作　　者：申进玲[1] 杨保伟[1] 席美丽[1] 孟江洪[1,2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]美国马里兰大学营养与食品科学系,美国马里兰州大学城md20742

出　　处：《食品科学》2011年第9期130-134,共5页Food Science

基　　金：教育部长江学者讲座教授奖励计划项目(Z111020001);西北农林科技大学留学回国人员基金项目(Z111020525)

摘　　要：采用PCR技术检测2007—2008年分离于陕西省的443份零售肉中63株沙门氏菌和29株奇异变形杆菌Ⅰ类整合子,得到其在菌株中的流行情况;使用限制性内切酶酶切和DNA序列分析技术确定Ⅰ类整合子所携带的耐药基因盒;采用国家临床实验室标准化委员会(CLSI)推荐的琼脂稀释法测定Ⅰ类整合子阳性菌株对14种抗生素的药敏性。结果表明:10株(16%)沙门氏菌含有Ⅰ类整合子,其大小主要为1281bp和1842bp两种类型,携带的耐药基因分别为IntI1-blaPSE-1和dhfrXII-orf-aadA2;7株(24%)变形杆菌含有Ⅰ类整合子,主要为1123、1526、1590bp 3种类型,携带的耐药基因分别有IntI1-blaPSE-1、aadB-aadA2和dfr17-aadA5。分离自陕西省零售肉中携带有多种Ⅰ类整合子的沙门氏菌和奇异变形杆菌均为多重耐药株,耐药基因分别介导对氨基糖苷类、β-内酰胺类和甲氧苄氨嘧啶类抗生素的抗性。奇异变形杆菌中Ⅰ类整合子检出率比沙门氏菌高,且种类较为丰富。Sixty-three Salmonella and twenty-nine Proteus mirabilis isolates were detected in retail meat from Shaanxi Province during the period of 2007—2008.Class Ⅰ integrons were identified by DNA sequence analysis and restriction enzyme digestion.The antibacterial susceptibility of class Ⅰ integron-positive strains were further tested by using fourteen antibiotics and the agar dilution method recommended by the Clinical Laboratory and Standard Institute（CLSI）.The results indicated that two kinds of class Ⅰ integrons were identified in 10 Salmonella isolates（16%）,with 1281 bp integron containing IntI1-blaPSE-1 and 1842 bp integron containing dhfrXII-orf-aadA2.Of twenty-nine Proteus mirabilis isolates,seven（24%） contained class Ⅰ integrons with 1123,1526 and 1590 bp harboring IntI1-blaPSE-1,aadB-aadA2 and dfr17-aadA5 genes.All class Ⅰ integron-postitive isolates were multi-drug resistant strains.The antibiotic resistant genes in these integrons could mediate resistance to β-lactams,trimethoprim and aminoglycosides.More class Ⅰ integrons existed in Proteus mirabilis than in Salmonella.

关 键 词：零售肉 沙门氏菌 奇异变形杆菌 整合子 耐药 

分 类 号：TS201.6[轻工技术与工程—食品科学]