海洋细菌NBRC10260产琼胶酶发酵条件优化  

Optimization of fermentation conditions for agarase production by a marine bacterium NBRC10260

作　　者：朱启忠[1] 朱慧文[1] 孙艳娜[1] 周晓龙[1] 郝梅[1] 郭振博[1] 施超[1] 

机构地区：[1]山东大学海洋学院,山东威海264200

出　　处：《中国酿造》2011年第5期53-55,共3页China Brewing

基　　金：国家创新性基金项目(101042239);山东大学科技立项重点项目专项基金(A10010)

摘　　要：通过正交试验法对产琼胶酶海洋菌株NBRC102603发酵条件进行优化,优化培养基为:蛋白胨4.0g/L、酵母粉1.25g/L、琼胶粉5.0g/L;在装液量150mL(500mL三角瓶)、28°C、150r/min、接种量为1%条件下发酵48h酶活力达到最高,为56.89U/mL,比未优化前提高了2.01倍。The fermentation conditions of an agarase-producing marine bacterium NBRC10260 were optimized by orthogonal test. The optimal culture medium consisted of 4. 0g/L peptone, 1.25g/L yeast extract and 5. 0g/L agar. The highest agarase activity （56. 89U/ml） was obtained when the fermentation was conducted in 500ml Erlenmeyer flask containing 150ml medium with shaking rate of 150r/min and inoeulum of 1% for 48h. The agarase activity was increased 3.01 times after optimization.

关 键 词：海洋细菌 琼胶酶 正交试验 

分 类 号：Q93-335[生物学—微生物学]