生物酶法合成植物甾醇酯的研究  

Study on synthesis of phytosterol esters using enzymatic method

作　　者：于殿宇[1] 王腾宇[1] 陈昊[2] 朱秀清[2] 江连洲[1,2] 胡立志[2] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030 [2]国家大豆工程技术研究中心,黑龙江哈尔滨150030

出　　处：《食品工业科技》2011年第6期318-319,393,共3页Science and Technology of Food Industry

基　　金：黑龙江省教育厅科学技术研究项目(11551064);黑龙江省2009科技攻关项目(WB09B201-1)

摘　　要：采用生物酶法对葵花油与植物甾醇的酯交换反应技术参数和条件进行了探索。采用Novozym435脂肪酶,催化植物甾醇与油脂的酯交换反应。通过单因素与正交实验,确定了最佳工艺参数:甾醇浓度5%,酶添加量1%,反应时间3h,反应温度100℃,搅拌速度600r/min,在最佳条件下酯交换反应的酯化率达到89.7%。Transesterification was conducted in sunflower oil with phytosterol by Novozym 435 lipase catalyzation.The optimum conditions were obtained by the single factor and orthogonal tests.Phytosterol concentration was 5%,the usage of lipase was 1%,the reaction time was 3h,the reaction temperature was 100℃,and the stirring speed was 600r/min.The esterification rate was 89.7% under the optimum conditions.

关 键 词：植物甾醇 葵花油 酯交换反应 脂肪酶 

分 类 号：TS201.1[轻工技术与工程—食品科学]