植物几丁质酶纯化、测定及应用研究进展  

Review on purification,enzyme assay and application of plant chitinases

作　　者：杨海霞[1] 邓建军[1,2] 张建[1,3] 赵广华[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]西北大学食品科学与工程系,陕西西安710069 [3]石河子大学食品学院,新疆石河子832000

出　　处：《食品工业科技》2011年第6期431-434,438,共5页Science and Technology of Food Industry

摘　　要：植物几丁质酶是植物体中能够水解几丁质多聚体的一种致病性相关蛋白(Pathogenesis-relatedproteins)。近年来对于几丁质酶的研究报道中,大量新型的植物几丁质酶被分离纯化,并建立了不同的酶活测定方法,在几丁质酶的结构及分类方面也逐步有了系统的研究。从几丁质酶的结构及分类,分离纯化以及已建立的酶活测定方法等方面取得的新进展进行了综述,并展望了几丁质酶在农业、食品生产及药用领域的应用前景。Plant chitinases which hydrolyze the chitin is one of pathogenesis-related proteins and can be induced in resistance of plants to fungal pathogens.Thus,plant chitinases has a wide range of application as a antisepticise material.Recently,chitinases from different plants have been purified and their enzymatic activities have been assayed with varied methods.The structures and classifications,different methods on enzymatic activity assay and purification were summarized.Moreover,applications of chitinases such as in food and medicine field were prospected.

关 键 词：植物 几丁质酶 分类 纯化 酶活测定 

分 类 号：TS201.25[轻工技术与工程—食品科学]