生物标记法剖析传统酿造用大曲微生物群落结构  

Characterization of Microbial Community Structure in Daqu Starter Based on Biomarkers

作　　者：秦臻[1] 郑佳[1] 彭昱雯[1] 金扬[1] 黄钧[1] 周荣清[1] 

机构地区：[1]四川大学轻纺与食品学院,制革清洁技术国家工程实验室,四川成都610065

出　　处：《食品科学》2011年第11期165-170,共6页Food Science

基　　金：四川省科技支撑计划项目(2008NZ0014);四川省重大科技攻关项目(09ZC00735)

摘　　要：建立一种基于生物标记物表征大曲类固态发酵体系微生物群落结构的生物化学方法。以微生物细胞膜的特征组分磷脂脂肪酸(PLFAs)组成信息为指标,描述大曲微生物群落结构特征;采用麦角甾醇标记物含量估算出样品真菌生物量。结果显示:5种不同生产工艺的大曲样品中共计检出18种磷脂脂肪酸,优势PLFAs是16:0、18:2ω6,9和18:1ω9,占总PLFAs物质的量的90%以上。从PLFAs组成特征判断5类大曲中优势菌群均为真菌。基于麦角甾醇含量与真菌生物量的关联性,估算出绝干大曲中的真菌生物量分布在(110.45±4.60)～(218.47±11.19)μg/mg。A biochemical technique for characterizing the microbial community structure in Daqu starter was established on the basis of different biomarkers.The microbial community structure was characterized by using the content of phospholipid fatty acids（PLFAs） determined by GC-MS and the biomass of predominant microbial community in Daqu starter was estimated by the content of ergosterol.A total of 18 species of PLFAs were determined quantitatively in Daqu starter.The predominant PLFAs were 16：0,18：2ω6,9 and 18：1ω9,which reached up to 90% of total PLFAs.The results showed that predominant microbial community in Daqu starter was fungi.According to the relative ratio between ergosterol content and fungal biomass in Daqu starter,fungal biomass in five kinds of Daqu starters was in the range of（110.45±4.60）-（218.47±11.19）μg/mg dry weight.

关 键 词：大曲 磷脂脂肪酸 微生物群落结构 麦角甾醇 真菌生物量 

分 类 号：Q599[生物学—生物化学]