玉米肽对小鼠酒后肝脏乙醇脱氢酶活力的影响及醒酒机理  

Effect of Corn Peptides on Alcohol Dehydrogenase Activity in Live of Mice after Drinking and Its Anti-alcohol Mechanism

作　　者：郭辉 何慧[1] 韩樱[1] 黄文浩[1] 张小波[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070 [2]安琪酵母股份有限公司,湖北宜昌443003

出　　处：《食品科学》2011年第11期265-269,共5页Food Science

基　　金：国家自然科学基金项目(30972043);国家"863"计划项目(2008AA10Z314)

摘　　要：为了研究玉米肽(CP)对大量摄入乙醇后小鼠的醒酒作用,采用气相色谱(GC)法测定小鼠血液中乙醇含量,NAD+法测定肝脏中乙醇脱氢酶(ADH)的活力,结合氨基酸分析,探讨其醒酒机理。结果显示:CP对肝脏中ADH有激活作用,且两者间存在明显剂量-效应关系,灌胃600mg/kg CP可极显著激活小鼠肝中ADH活性(P<0.01),激活率达30.1%;并极显著抑制血清中乙醇含量的提高(P<0.01),小鼠血清中乙醇含量的降低与CP间亦存在明显的剂量-效应关系。在小鼠灌胃乙醇后20～200min内,给CP组血醇清除率和ADH活力均明显高于乙醇模型组;在小鼠灌胃乙醇20～120min内,给CP组与模型组血醇清除率差异显著(P<0.05或P<0.01);在小鼠灌胃乙醇50～120min内,ADH活力差异显著(P<0.05)。氨基酸分析表明,玉米肽具有较高的疏水性。用85%乙醇洗脱的疏水性最强的组分对羟自由基的抑制率最高,达83.05%。结论:玉米肽能激活ADH,且有良好的持续激活作用,其作用可能与玉米中疏水性短肽有关。In order to explore the anti-alcohol mechanism of corn peptides（CP） on heavy-drinking mice,blood alcohol concentration（BAC） was determined by gas chromatography（GC）,ADH activity in liver was detected by NAD＋ method and the amino acid composition of CP was analyzed.The results showed that CP could activate ADH in liver in a dose-dependent way.CP at the dose of 600 mg/kg·bw could significantly increase ADH activity in mice administered with alcohol（P 0.01）,result in an ADH activation rate of up to 30.1%,and remarkably reduce BAC in mice（P 0.01）.The scavenging rate of BAC was positively correlated with the dose of CP.The scavenging rate of BAC and the ADH activity in mice from CP group were remarkably higher than that in mice from alcohol model group after the administration of alcohol for 20-200 min.Compared with the model group,the scavenging rate of BAC in mice from the CP group was significantly different during alcohol administration for 20-120 min（P 0.05 or P 0.01）.Amino acid analysis showed that CP had high hydrophobicity.The highest hydrophobic fraction was the most potent hydroxyl radical scavenger with scavenging rate of 83.05%.Therefore,CP can continuously activate ADH in liver,which likely to be related to hydrophobic short peptides in corn.

关 键 词：玉米肽 醒酒 乙醇脱氢酶 血液乙醇含量 疏水性短肽 

分 类 号：Q514.3[生物学—生物化学]