捷安肽素固定化细胞分批发酵过程动力学分析  

Batch Fermentation Kinetic Analysis of Jiean-Peptide Production by Immobilized Cells

作　　者：张晓勇[1,2] 钟娟[1] 李志东[1] 周金燕[1] 杨杰[1] 肖亮[1] 谭红[1] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041 [2]中国科学院研究生院,北京100049

出　　处：《高校化学工程学报》2011年第3期459-464,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-YW-G-039-2;KSCX2-EW-G-16);国家科技支撑计划课题(2011BAE06B04)

摘　　要：用Sigmoid模型构建了5 L发酵罐固定化细胞分批发酵生产捷安肽素的非结构动力学模型,并对细胞生长、捷安肽素合成、葡萄糖消耗和总氮消耗的比速率进行了计算与分析。结果表明,Sigmoid模型能较好地拟合固定化细胞分批发酵生产捷安肽素的发酵过程,拟合动力学模型的相关系数R2大于0.971。细胞生长、捷安肽素合成、葡萄糖消耗和总氮消耗的最大比速率分别为0.411、0.001、0.771和0.043 h-1。细胞和捷安肽素对葡萄糖的最大得率系数分别为2.1和0.029 g·g-1;细胞和捷安肽素对总氮的最大得率系数分别为51和3.4 g·g-1。细胞生长与捷安肽素的合成呈部分偶联关系。Using Sigmoid model the unstructured kinetics of Jiean-peptide（JAA） batch fermentation process in 5 L bioreactor by immobilized bacillus subtilis cells was investigated,and the specific rates of cell growth and JAA formation,glucose consumption and total nitrogen consumption were analyzed and calculated.The results show that the Sigmoid model can match well with the JAA batch fermentation process in 5 L bioreactor by immobilized bacillus subtilis cells,and the squares of correlation corresponding to Sigmoid model exceed 0.971.The maximum specific rates of cell growth,JAA formation,glucose consumption and total nitrogen consumption are 0.411,0.001,0.771 and 0.043 h-1,respectively.The maximum yields coefficient of cell and JAA from glucose is 2.1 and 0.029 g·g-1,respectively,and the maximum yields coefficient of cell and JAA from total nitrogen is 51 and 3.4 g·g-1,respectively.The relation between the cell growth and JAA formation is a relation combining growth-associated and nongraoth-associated processes.

关 键 词：捷安肽素 固定化细胞 分批发酵 发酵动力学 枯草芽孢杆菌 

分 类 号：TQ921.7[轻工技术与工程—发酵工程] TQ920.1