甲壳质清洁生产中酸性蛋白酶脱蛋白的研究  

A Study of Clean Chitin Production by Acid Protease

作　　者：叶夏青[1] 刘必谦[1] 唐杰[1] 庄怡[1] 

机构地区：[1]宁波大学教育部应用海洋生物技术重点实验室,宁波315211

出　　处：《食品工业》2011年第6期56-58,共3页The Food Industry

摘　　要：甲壳质开发应用领域异常广阔,国内外需求量大。我国目前多采用化学法,即酸碱法来生产虾壳甲壳质,生产中产生大量难以处理的污染物。用宇佐美曲霉酸性蛋白酶脱蛋白来生产甲壳质基本上不产生污染,水洗废液还可以进行回收利用。采用宇佐美曲霉酸性蛋白酶进行了甲壳质清洁生产中脱蛋白的研究,比较了温度、加酶量和pH等因素对脱蛋白的影响。结果表明:当温度为40℃、加酶量为0.4%、pH3.0时,脱蛋白率达到60%,蛋白与虾壳可以完全分离。Chitin is green material with unique characteristics; it has good organizational compatibility, it＇s biodegradable and also environmentally friendly. Its exploitation has an extremely vast realm and therefore great potential applications.Wastes from conventional chitin production pollute marine environment and ecology seriously.A new and green procedure using Aspergillus usamii acid protease was developed in which few or nil waaste was released.Factors affecting the deproteinization were temperature, enzyme concentration and pH were test.The results indicate that protein and shrimp shell can be completely separated at the conditions： temperature at 40 ℃, enzyme concentration at 0.4%, pH at 3.0.Moreover, all the protein,peptides,amino acid,fat,astacin,calcium lactate could be reused for recycle.therefore,the acid protease fermentation method is an environment friendly and efficient one to produce chitin from shrimp shell waste and could be applied in an industrial scale.

关 键 词：宇佐美曲霉 酸性蛋白酶 脱蛋白:甲壳素 甲壳质清洁生产 

分 类 号：TQ914.2[化学工程]