基于DO-stat流加培养控制的L-异亮氨酸发酵条件优化  

Optimization of L-isoleucine Fermentation Conditions Based on DO-stat Feeding Culture Strategy by Brevibacterium lactofermentation

作　　者：张俊丽[1,2] 刘龙[1,2] 房峻[1,2] 李江华[1,2] 堵国成[1,2] 陈坚[3] 宁健飞 蔡立明 

机构地区：[1]江南大学生物工程学院,无锡214122 [2]江南大学工业生物技术教育部重点实验室,无锡214122 [3]江南大学食品科学与技术国家重点实验室,无锡214122 [4]无锡晶海氨基酸有限公司,无锡214199

出　　处：《应用与环境生物学报》2011年第3期317-320,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家"973"计划项目(No.2007CB714306);"973"前期研究专项(No.2010CB535014);国家杰出青年基金项目(No.20625619);国家自然科学基金重点项目(No.20836003);国家"863"计划项目(No.2007AA02Z229);中央高校基本科研业务费专项(No.JUSRP30901)~~

摘　　要：L-异亮氨酸(L-Ile)产生菌代谢流的前期研究报道认为,较低的溶氧浓度有利于L-异亮氨酸的积累.为进一步提高L-异亮氨酸的产量,采用DO-stat流加培养控制方法,分别研究了溶氧浓度20%、30%、35%和40%下乳糖发酵短杆菌发酵液中葡萄糖浓度的变化以及L-异亮氨酸的合成情况.结果表明,当发酵罐中溶氧浓度控制在20%时,L-异亮氨酸的产量为24.3 g L-1,比分批发酵提高了15.7%.其中,生产强度为0.338 g L-1 h-1,底物转化率为12.2%.The dissolved oxygen（DO） concentration plays a significant role in the accumulation of amino acids during amino acid fermentation process.According to the known study on the metabolic flux of L-isoleucine producing strain,lower DO concentration was considered more desirable for L-isoleucine production.In this study,DO-stat feeding strategy was adopted to increase L-isoleucine production.Changes in glucose concentration and L-isoleucine formation under different DO concentrations,i.e.,20%,30%,35% and 40% were investigated.The result demonstrated that 24.3 g L-1 L-isoleucine with productivity of 0.338 g L-1 h-1 and substrate conversion ratio of 12.2% was achieved when DO concentration was controlled at 20%,which was 15.7% higher than that of batch culture.Fig 3,Tab 1,Ref 25

关 键 词：L-异亮氨酸 溶氧(DO) DO-stat 补料分批发酵 乳糖发酵短杆菌 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]