烟曲霉菌株发酵产壳聚糖酶条件的优化  

Fermentation optimization for the production of chitosanase by Aspergillus fumigatus

作　　者：齐辉连[1] 陈桂光[1] 李玮[1] 张云开[1] 梁智群[1] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室微生物及植物遗传工程教育部重点实验室,广西大学生命科学与技术学院,广西南宁530004

出　　处：《中国酿造》2011年第6期148-152,共5页China Brewing

摘　　要：通过单因子试验和正交试验对选育的一株产壳聚糖酶菌株UC-233进行发酵条件优化,结果表明其最适氮源是黄豆粕粉,最适碳源是壳聚糖,其最适发酵条件是:黄豆粕粉3.3%,胶体壳聚糖1.5%,培养基初始pH为4.0,250mL三角瓶装液量为50mL,接种量为1mL,发酵温度是30℃,发酵时间为48h,在此条件下菌株发酵液的壳聚糖酶活力达39.5U/mL,是优化前的1.43倍。Optimization ofUC-233 fermentation conditions was studied via single factor and orthogonal test design. The results showed that： the optimum nitrogen source was soy meal powder, the optimum carbon source was colloid chitosan, the optimized fermentation conditions for chitosanase production were： soy meal powder 3.3%, colloid chitosan 1.5%, initial pH value 4.0,250ml flask containing 50ml medium, inoculation quantity lml, temperatm＇e was 35℃, fermentation time was 48h,Under these conditions, the highest chitosanase activity could reach 39.5U/ml, which was 1.43 times compared with that before optimization.

关 键 词：壳聚糖酶 发酵条件 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程]