高效液相色谱法测定蛋鸭组织中的类胡萝卜素  

Analysis of Carotenoids in Laying Duck Tissues Using High Performance Liquid Chromatography

作　　者：薛峰[1] 李晨[1] 江沛[1] 王多[1] 任真明[1] 潘思轶[1] 王可兴[1] 

机构地区：[1]华中农业大学食品科学技术学院,湖北武汉430070

出　　处：《食品科学》2011年第14期185-189,共5页Food Science

基　　金：国家自然科学基金面上项目(30972044)

摘　　要：通过响应面分析法对蛋鸭肝脏组织中类胡萝卜素的提取方法进行优化。利用响应面试验设计考察提取时间、提取温度、皂化液质量浓度对总类胡萝卜素提取率的影响。结果表明:蛋鸭肝脏组织中类胡萝卜素提取最优条件为提取时间90min、提取温度37℃、皂化液质量浓度50g/100mL;建立的采用反相高效液相色谱测定蛋鸭肝脏组织和血清中类胡萝卜素的方法的检出限为20ng/mL,在肝脏组织和血清样品中的加标回收率分别为95.21%和105.50%,相对标准偏差分别为5.71%和6.73%。With the aim of developing an optimal sample preparation method for the determination of carotenoids in laying duck liver tissues by high performance liquid chromatography, response surface methodology was employed to optimize the extraction recovery of carotenoids from laying duck liver tissues with respect to three extraction conditions such as temperature, extraction time and KOH concentration. The optimal extraction conditions were achieved as follows： extraction time 90 min, temperature 37 ℃ and KOH concentration 50 g/100 mL. The HPLC method established based ou these extraction conditions exhibited a detection limit of 20 ng/mL for carotenoids in the liver and serum laying duck. The mean spike recovery rates for carotenoids in the liver tissues and serum laying duck were 95.21% and 105.50% with a relative standard deviation （RSD） of 5.71% and 6.73%, respectively.

关 键 词：蛋鸭 肝脏 血清 

分 类 号：Q584[生物学—生物化学]