制备高不溶性大豆膳食纤维得率的菌种选育  

Breeding strain of high conversion ratio of IDF by mutation

作　　者：刘娴[1] 朱道林[1] 林胜[1] 周逢旭[1] 戴永平[1] 王池丽[1] 宫震[1] 

机构地区：[1]巢湖职业技术学院生物应用技术系,巢湖238000

出　　处：《食品科技》2011年第7期11-15,共5页Food Science and Technology

基　　金：安徽省高等学校自然科学研究基金项目(KJ2010B129);巢湖职业技术学院自然科学研究基金项目(zrkx200805)

摘　　要：不溶性膳食纤维是一种新型的功能性食物配料,在功能食品中应用广泛,且在预防人体的某些疾病方面起着重要作用,所以如何在保持膳食纤维高活性的基础上提高其转化率显得尤为重要。以生产豆制品的废渣为原料,以保加利亚乳杆菌(Lb.)和嗜热链球菌(St.)为混合菌种,首先对这2种菌进行物理(紫外线)和化学(硫酸二乙酯)单因素诱变,确定2种菌的紫外线照射时间均为1.5min,硫酸二乙酯的振荡时间均为30min。在此基础上进行复合诱变,筛选得到不溶性大豆膳食纤维得率为84.95%和83.63%的菌种各1株,较原菌分别提高了1.47%和1.80%。同时其溶胀性和持水力也得到了一定程度的提高。Insoluble dietary fiber is a kind of new functional food ingredients,widely used in functional food,and the body in certain disease prevention plays an important role in maintaining,so how high dietary fiber based on improving its conversion is particularly important.Through mutating two acidophilus milk production strains（Lactobacillus delbrukeii subsp.bulgaricus and Streptococcus salivarius subsp.thermophillus）,two high conversion ratio of reducing sugar were obtained respectively.Firstly,the ultraviolet ray（UV） and the diethyl sulfate（DES） were used to dispose these two strains separately,then selected the optimum mutation condition：the irradiation of ultraviolet ray 1.5 minute and 30 minute of DES disposal.Then disposed each strain which had been mutated by UV of 1.5minute with DES of 30 minute severally,and obtained two new strains which the conversion ratio of IDF were 84.9% and 83.6%,increased 1.43% and 1.83%.At the same time,the water retention capability and the expansibility ossibility were increased greatly.

关 键 词：大豆 选育 不溶性膳食纤维得率 持水力 溶胀性 

分 类 号：TS202.1[轻工技术与工程—食品科学]