恶臭假单胞菌透性化细胞海藻糖合酶的酶反应工艺及其特性研究  

Characteristics of Trehalose Synthase from Permeablized Pseudomonas putida Cells and Its Enzymatic Reaction Process

作　　者：徐汝意[1] 马春玲[1] 王腾飞[1] 李丕武[1] 王瑞明[1] 

机构地区：[1]山东省微生物工程重点实验室山东轻工业学院食品与生物工程学院,山东济南250353

出　　处：《酿酒科技》2011年第7期23-26,共4页Liquor-Making Science & Technology

基　　金：山东省高新技术自主创新工程专项计划项目(2008Zz29)

摘　　要：用甲苯对恶臭假单胞菌细胞进行透性处理,研究了透性化细胞海藻糖合酶的酶反应工艺及其特性。研究结果表明,该细胞酶的最适反应条件为pH7.8,转速180 r/min,温度为40℃,反应时间为8 h。透性化细胞海藻糖合酶具有极强的底物特异性,激活离子有Na+、K+,重金属离子强烈抑制菌体生长和酶活。Trehalose is a novel functional disaccharide,produced mainly by enzyme method nowadays,while trehalose synthase is a novel enzyme which catalyzes maltose into trehalose by one step.In this study,the characteristics of trehalose synthase from permeablized Pseudomonas putida cells and its enzymatic reaction process after permeated by toluene were investigated.The optimum reaction conditions were summed up as follows： pH=7.8,rotating speed=180 r/min,temperature at 40 ℃,and reaction time=8 h.The activity of trehalose synthase could be enhanced by the addition of Na＋ and K＋,but be inhibited by heavy metal ion.Meanwhile,trehalose synthase presented extremely strong substrate specificity.

关 键 词：透性 海藻糖合酶 恶臭假单胞菌 酶学特性 

分 类 号：TQ925[轻工技术与工程—发酵工程]