牡丹嫁接品种活性成分的测定及抗氧化活性的研究  

Study on active substances and antioxidant capacities of tree peony graft varieties

作　　者：黄海霞[1] 刘领[1] 齐欢欢[1] 张静[1] 张志新[1] 宋兵兵[1] 

机构地区：[1]河南科技大学农学院,河南洛阳471003

出　　处：《食品工业科技》2011年第8期147-149,共3页Science and Technology of Food Industry

基　　金：河南科技大学SRTP项目(2008142)

摘　　要：[目的]旨在探究嫁接后牡丹体内活性物质的含量和抗氧化活性的变化,为开发牡丹在天然抗氧化剂和医药保健等相关领域的应用提供一定的理论和实验基础。[方法]本研究以洛阳红、凤丹白和洛阳红嫁接品种、凤丹白嫁接品种的牡丹根为材料,研究了95%乙醇提取物在1,1-二苯-2-苦味肼(DPPH)自由基体系、卵黄脂蛋白不饱和脂肪酸(PUFA)体系、邻苯三酚自氧化体系中的抗氧化活性,对比分析不同品种牡丹的抗氧化效果。[结果]嫁接品种提取液中芍药苷、牡丹酚含量均明显低于其他两个牡丹品种;其中,"凤丹白"的活性成分含量最为丰富。嫁接品种提取液抗卵黄脂蛋白PUFA过氧化的效果明显优于其他两个牡丹品种,在高浓度下清除DPPH自由基的能力较强,且其抗氧化能力与提取液浓度之间呈现出良好的剂量依赖效应,但其对O2-·的清除能力却明显弱于其他两个牡丹品种。[结论]牡丹根提取液有较好的抗氧化能力,嫁接后,牡丹体内活性成分含量及抗氧化活性等方面均有一定程度的变化。[Objective] Aimed to probe into the content of the active substances and anti-oxidant activities in the tree peony after grafting,the experimental and theoretic basis for the exploitation of tree peony in natural antioxidant and medical treatment and health protection was provided.[Method] The antioxidant activities of the 95% ethanol extracts from the root of four tree peony like Luoyanghong,Fengdanbai and their graft varieties were measured in vitro by the assays of 1,1-diphenyl-2-picrylhydrazyl（DPPH）radical,peroxidation of unsaturated fatty acid（PUFA）in lipoprotein,as well as the pyrogallol auto-oxidation.The antioxidant activities of several tree peony extracts were analyzed and compared.[Result] Contents of paeoniflorin and paeonolum in the extracts of graft varieties were obviously lower than other two tree peony varieties.Among them,＂fengdanbai＂ had the most abundant active substances.The extracts of graft varieties in which the inhibiting effects on anti-peroxidation of unsaturated fatty acid in lipoprotein were obviously superior to other two tree peony varieties and the scavenging effects on DPPH radical were better under the high concentration.And the antioxidant effects were enhanced with the concentration of extracts increasing.But the scavenging effects of the graft varieties on super oxide anion radical markedly were weaker than other two tree peony.[Conclusion] Several tree peony extracts had the better antioxidant capacities.The contents of the active substances and anti-oxidant activities in the graft varieties had great changes.

关 键 词：牡丹 嫁接 抗氧化能力 1 1-二苯-2-苦味肼自由基 不饱和脂肪酸 

分 类 号：TS201.1[轻工技术与工程—食品科学]