黑曲霉低聚异麦芽糖高产菌株的诱变选育  

Mutation breeding of Aspergillus niger for enhancement of isomaltooligosaccharides production

作　　者：陈桂光[1] 李玮[2] 齐辉连[1] 张云开[1] 梁智群[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005 [2]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《食品工业科技》2011年第8期185-187,327,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(20362001);国家"863"项目(2006AA10Z339)

摘　　要：以黑曲霉GXM-3为出发菌株进行60Co-γ射线与紫外线照射复合诱变育种,通过苔盼兰平板筛选及摇瓶培养,获得42株转化木薯淀粉液化液生成低聚异麦芽糖浆能力较强的突变株。其中,突变株D-597的转化能力最强。其在3L发酵罐中发酵80h,糖浆产物中低聚异麦芽糖含量达到最高值169.4mg/mL,比出发菌株提高了37.2%,发酵周期则缩短了40h。突变株D-597在利用木薯淀粉生产低聚异麦芽糖方面具有一定的工业应用前景。Aspergillus niger GXM-3 was mutagenized using Co60-γ rays and ultraviolet rays irradiation.42 mutants which converted liquefied cassava starch to isomaltooligosaccharides（IMO）syrup more efficiently were obtained by Trypan blue method and shake flask culture.Among them,mutant D-597 showed the highest activity,its peak yield（169.4mg/mL）of IMO in 3L fermenter occurred at 80h of the cultivation.Compared with the original strain,IMO productivity increased by 37.2% and fermentation period shortened by 40h.Mutant D-597 had application potential in industrial IMO production.

关 键 词：黑曲霉 低聚异麦芽糖 诱变育种 Α-葡萄糖苷酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]