丹贝异黄酮提取物的毒理学实验研究  

Toxicological Study on Isoflavones in Tempe

作　　者：蒋兆坤[1] 翟成凯[1] 孙桂菊[1] 陆琮明[1] 张小强[1] 高霞[2] 江汉湖[2] 

机构地区：[1]南京铁道医学院公共卫生学院,南京210009 [2]南京农业大学食品科技学院,南京210095

出　　处：《南京铁道医学院学报》1999年第4期219-221,共3页Journal of Nanjing Railway Medical College

基　　金：国家自然科学基金!资助课题(39470610)

摘　　要：目的:对丹贝异黄酮提取物进行毒理学安全性评价。方法:通过急性毒性试验以及致突变试验对丹贝异黄酮提取物进行毒理学评价。结果:(1) 急性毒性试验显示丹贝异黄酮提取物的LD50 大于每公斤体重21 .5 g( 含异黄酮376 mg);(2)Ames 试验、骨髓微核试验、精子畸形试验均为阴性结果。结论:(1) 丹贝异黄酮提取物LD50 大于每公斤体重21.5g,说明该样品无毒,但不能作为判定其安全性的唯一依据,仅供进一步毒理试验参考;(2)Objective:The aim of this study was to estimate the toxicological characterization of isoflavones in tempe.Methods:The acute toxicity test,Ames test,micronucleus test of bone marrow cell,and sperm shape abnormality test in mice were used in study.Results:(1)The acute toxicity test showed that the LD 50 of isoflavones in tempe was more than 21.5 g/kg(376 mg of isoflavones content).(2)The result of Ames test,micronucleus test of bone marrow cell,and sperm shape abnormality test in mice was negative.Conclusions:(1)The acutetoxicity test showed that the isoflavones in tempe had no toxicity,but the toxicity can not be judged with this test only.The data of acute toxicity test may be used for following toxicological test.(2)The isoflavones in tempe can't cause mutation.

关 键 词：丹贝 异黄酮 毒理学 小鼠 实验 大豆发酵食品 

分 类 号：R155.5[医药卫生—营养与食品卫生学]