大豆分离蛋白生产降血压肽酶解技术研究  

Producing Technology of Antihypertensive Peptide Derived from Soybean Protein Isolated by Enzymatic Method

作　　者：胡少新[1] 江连洲[2] 许德春[1] 赵晓南[1] 付立新[1] 孟丽芬[1] 潘媛媛[3] 

机构地区：[1]黑龙江省农业科学院玉米研究所,黑龙江哈尔滨150086 [2]东北农业大学食品学院,黑龙江哈尔滨150030 [3]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2011年第4期643-647,共5页Soybean Science

基　　金：农业部大豆产业技术体系资助项目(nycytx-004)

摘　　要：选用6种蛋白酶水解大豆分离蛋白,根据ACE抑制率高低,最终选择胰蛋白酶为最佳蛋白酶。通过单因素试验和L16(45)正交试验研究底物浓度、水解时间、酶与底物比、温度和pH值对酶解液降血压活性的影响,确定了水解最优条件组合。结果表明:底物浓度5%,水解时间6 h,酶和底物比为0.08,温度为50℃,pH值为8.0时,所得水解液ACE抑制率最高,为76.8%。Trypsinase was selected from six proteinases according to the angiotensin converting enzyme（ACE） inhibitory rate.Single factor experiments and orthogonal design L16（45） were adopted to investigate the effect of substrate concentration,hydrolysis time,E/S,temperature and pH value on ACE inhibitory activity of the product.The optimum trypsinase hydrolysis process for producing ACE inhibitory peptides was under 50℃,with pH 8.0,5% substrate concentration,0.08 E/S for 6 hours.After optimization,the ACE inhibitory activity reached 76.8%.

关 键 词：大豆分离蛋白 ACE抑制肽 酶解 ACE抑制率 

分 类 号：S565.1[农业科学—作物学]