芦笋下脚料中黄酮类物质分离纯化及鉴定  

Purification and Identification of Flavonoids from Asparagus' Waste Materials

作　　者：孙长花[1] 王正云[2] 钱小丽[3] 张素华[4] 

机构地区：[1]扬州职业大学生化工程系,扬州225000 [2]江苏畜牧兽医职业技术学院食品科技系,泰州225300 [3]江苏省扬州商务高等职业学校,扬州225003 [4]扬州大学食品科学与工程学院,扬州225127

出　　处：《食品工业》2011年第8期51-53,共3页The Food Industry

摘　　要：通过研究大孔吸附树脂的吸附和解吸性能,确定了AB-8型树脂分离纯化芦笋下脚料中黄酮类物质的条件为:吸附流速2mL/min,洗脱流速1.5mL/min;吸附原液pH6,洗脱剂为体积分数大于70%乙醇溶液,洗脱剂用量为200mL。纯化后的黄酮类物质经颜色反应和紫外光谱鉴定,主要为黄酮醇类。Through the comparison macroporous resin＇s adsorption and desorption capacity of flavonoids from asparagus,waste materials,the optimal condition of AB-8 resin for separating and purificating flavonoids was determined as follows：the adsorption velocity was 2 mL/min,elution velocity was 1.5 mL/min,the pH value of under-adsorpted solution was 6,the elution solvent was the volume fraction more than 70% ethanol solution,and the quantity of elution solvent was 200 mL.Finally,the flavonoids of purification product which was detected by color reaction and spectrum could belongs to flavonol.

关 键 词：芦笋 黄酮类物质 纯化 鉴定 

分 类 号：TQ461[化学工程—制药化工]