复合诱变选育茁霉多糖高产菌株  

Screening of a strain with high pullulan productivity by compound mutagenesis

作　　者：冯印[1] 苏安祥[1] 王玉华[1] 

机构地区：[1]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《中国酿造》2011年第8期84-86,共3页China Brewing

基　　金：吉林省科技厅科技支撑计划(20080251)

摘　　要：茁霉多糖具有许多优良的化学性质,可以作为中间体合成多种有经济价值的工业用化合物,是一种潜在的重要化工平台产品。以出芽短梗霉Y为出发菌株,通过紫外2轮、亚硝基胍3轮复合诱变,高蔗糖浓度(20%)平板筛选得到6株高产突变株,其中菌株N3茁霉多糖产量质量浓度达到37.84g/L,比出发菌株产量提高71.22%。Pullulan has many excellent chemical properties, which can be used as an intermediate to synthesize a variety of industrial compounds. Aureobasidium puHulans was treated by UV for two generation and NTG for three generation mutagenesis. Six mutant stains were screened by high sucrose concentration （20%） plate, in which a mutant strain N3 with high pullulan productivity was obtained. The product of pullulan by this strain was up to 37.84g/L, which is 71.22% higher than that of the parent strain.

关 键 词：茁霉多糖 紫外 亚硝基胍诱变 

分 类 号：Q93-331[生物学—微生物学]