邻二氮菲-Fe^(3+)法测定保健食品中水溶性成分的抗氧化能力  

Determination of Antioxidant Capacity of Water-soluble Compounds in Functional Food by Phenanthroline-Ferric Ion Method

作　　者：刘薇[1] 刘雯佳[1] 刘彦霞[1] 赵建[1] 文镜[1] 

机构地区：[1]北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《食品科学》2011年第16期261-264,共4页Food Science

基　　金：北京市教委科技发展计划项目(KM201011417006)

摘　　要：目的:建立用邻二氮菲-Fe3+测定保健食品中水溶性成分抗氧化能力的方法。方法:用水相提取保健食品中的水溶性抗氧化成分,使其还原反应体系中的Fe3+,生成的亚铁离子与邻二氮菲反应成稳定的橙红色络合物,用分光光度计在536nm波长处测定吸光度。以水溶性VE为对照,由此计算样品水溶性成分中抗氧化(还原性)物质的多少。通过对方法线性关系、最低检测限、精密度、准确度等指标进行测定,验证方法的可行性。结果:检测Trolox在质量浓度0.66～10.53μmol/L范围内呈现良好的线性关系(R2=0.9999);最低检测下限为0.54μmol/L;平行性精密度为2.7%;重复性精密度为3.3%;5次重复加标回收率为(99.9±4.6)%。结论:邻二氮菲-Fe3+法可用于保健食品中水溶性成分抗氧化能力的检测。Objective： To establish a colorimetric method to determine the antioxidant capacity of water-soluble compounds in functional food based on the reaction between 1,10-2 phenanthroline and Fe3＋ to form yellow complexes.Methods： The hydrophilic antioxidants in functional foods were extracted with pH 7.4 PBS buffer solution（75 mmol/L） and allowed to reduce Fe3＋ to Fe2＋.Fe2＋ was exposed to 1,10-2 phenanthroline to form a stable Fe2＋-phenanthroline complex.The absorbance of the complex was determined on a spectrophotometer at 536 nm and used to calculate the antioxidant capacity of water-soluble compounds in functional food using Trolox,a water soluble vitamin E analog,as the reference standard.The accuracy,linearity,repeatability,recovery rate and limit of detection（LOD） of this method were studied,and its feasibility was also verified.Results： This method showed a good linearity over the Trolox concentration of 0.66-10.53 μmol/L（R2 = 0.9999）.The LOD was 0.54 μmol/L.The replicability and repeatability was 2.7% and 3.3% relative standard deviation（RSD）,respectively.The mean spike recovery was（99.9±4.6）%（n = 5）.Conclusion： This method is applicable to determine the antioxidant capacity of water-soluble compounds in functional food.

关 键 词：保健食品 抗氧化能力 邻二氮菲-Fe3+法 

分 类 号：TS207.3[轻工技术与工程—食品科学]