蛋氨酸羟基类似物对小鼠组织自由基和抗氧化作用研究  

Research of 2-hydroxy-4-(methylthio)butanoic acid on free radicals of tissues and antioxidation of mice

作　　者：杨永兰[1] 夏淑芳[1] 刘骥[1] 乐国伟[1,2] 施用晖[1,2] 

机构地区：[1]江南大学食品学院,食品营养与功能因子研究中心,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品工业科技》2011年第9期399-402,453,共5页Science and Technology of Food Industry

基　　金：国家自然科学基金(30571347)

摘　　要：目的:研究蛋氨酸(MET)和蛋氨酸羟基类似物(HMTBA)对饲喂高脂日粮小鼠自由基水平、抗氧化作用及其血脂代谢的影响。方法:50只昆明种雄性小鼠分成5组,分别是正常+0.2%MET组、正常+0.1%MET+0.1%HMTBA组、高脂+0.2%MET组、高脂+0.2%HMTBA组、高脂+0.1%MET+0.1%HMTBA组。结果:与正常+0.2%MET组相比,高脂+0.2%MET可导致小鼠氧化应激,升高血液和组织的自由基水平,降低机体抗氧化能力,使血脂水平和动脉粥样硬化指数升高;而高脂+0.2%HMTBA能显著降低机体自由基水平,增强血清及组织中GSH-Px及CAT活性,显著升高T-AOC水平,显著降低MDA水平,与高脂+0.2%MET组相比,HDL升高,LDL、TG、TC、AI显著降低。结论:两种蛋氨酸源中,HMTBA有较好地抗氧化作用,能够更好地清除体内的自由基,提高机体的抗氧化能力,降低血脂。Objective：To research the effects of methionine sources methionine（MET）and 2-hydroxy-4-（methythio）butanoic acid（HMTBA）on the level of free radicals and antioxidation in mice fed with high-fat diet.Method：Fifty mice were randomly divided into 5 groups：low-fat with 0.2% MET group,low-fat with 0.1% HMTBA and 0.1%MET group,high-fat with 0.2% MET group,high-fat with 0.2% HMTBA group,high-fat with 0.1% HMTBA and 0.1% MET group.Results：Compared with low-fat with 0.2% MET group,high-fat with 0.2% MET group can induce oxidative stress,enhance the level of free radicals in whole blood and tissues significantly,decrease the antioxidant activity,enhance blood lipid level and atherosclerosis index.But high-fat with 0.2% HMTBA group could decrease the level of free radicals in vivo significantly,enhance the activity of GSH-Px,CAT,T-AOC of the serum and tissues significantly,decrease the level of MDA,compared with hight-fat with 0.2% MET group,HDL level was higher and LDL,TG,TC,AI levels were lower significantly.Conclusion：HMTBA can better scavenge free radicals and increase the antioxidative capacity and reduce the blood lipid in mice fed with high diet in two methionine sources.

关 键 词：蛋氨酸 蛋氨酸羟基类似物 高脂日粮 抗氧化作用 降血脂 

分 类 号：TS201.6[轻工技术与工程—食品科学]