超高效液相色谱-串联质谱法同时测定鱼、肉类罐头食品中的BADGE、NOGE及其衍生物  

Determination of Bisphenol a Diglycidyl Ether(BADGE),Novolac Glycidyl Ethers(NOGE) and Its Derivatives in Canned Fish and Meat by Ultra-performance Liquid Chromatography with Tandem Mass Spectrometry

作　　者：林赛君[1] 陈思[2] 薛鸣[3] 张虹[2] 

机构地区：[1]杭州市质量技术监督检测院,浙江杭州310014 [2]浙江工商大学食品与生物工程学院,浙江杭州310035 [3]杭州市疾病预防控制中心,浙江杭州310021

出　　处：《食品与发酵工业》2011年第8期155-160,共6页Food and Fermentation Industries

基　　金：浙江省科技厅项目(2010C32G2050021)资助;浙江省自然科学基金项目(Y3100689)资助;浙江省质量技术监督系统科研计划项目(20100221)资助

摘　　要：建立了一种罐头食品中的BADGE(bisphenol A diglycidyl ether,双酚A-二环氧甘油醚)、NOGE(novolacglycidyl ether,酚醛清漆甘油醚)及其衍生物含量的超高效液相色谱-串联质谱检测法。前处理包括正己烷/丙酮微波辅助萃取,Varain-PS-DVB固相萃取柱净化等。样品经BEH C18色谱柱(100 mm×2.1 mm,1.7μm)分离,以乙腈和0.2%甲酸水为流动相,进行梯度洗脱,在API 4000-QTRAP质谱仪电喷雾正离子、多反应监测(MRM)模式下进行检测。分析物检出限达到0.010 2 ng/g到0.197 2 ng/g,三水平加标回收率在65.7%～99.1%。An ultra-performance liquid chromatography with tandem mass spectrometry method for determination of bisphenol A diglycidyl ether （BADGE） , novolac glycidyl ether （NOGE） and its derivatives from canned food has been developed. The proposed method includes microwave-assisted extraction followed by puri？ cation by solid phase extraction （SPE）. After separation by BEH Cls （ column 100 mm ×2.1 mm, 1.7 μm）, the sample was detected by API 4000 - QTRAP mass spectrometry under positive electrospray ionization mode, with acetonitrile and 0.2% aqueous formic acid as mobile phase. The detection limits were 0. 010 2 - 0. 197 2 ng/g, and recoveries at three different concentrations ranged from 65.7% to 99. 1%. This method was successfully applied to canned fish and meat, the highest total contents of 3 -6 R-NOGE reached 209.03 mg/6 dm2.

关 键 词：超高效液相色谱-串联质谱法 罐头食品 BADGE NOGE 衍生物 检测 

分 类 号：TS297[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]