海洋壳聚糖酶高产菌株的筛选和鉴定  

Screening and Identification of a High-yield Chitosanase-producing Strain from Ocean

作　　者：朱明[1,2] 孙玉英[1] 张继泉[3] 莫照兰[3] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]江苏省海洋资源开发研究院,江苏连云港222001 [3]中国科学院海洋研究所,山东青岛266071

出　　处：《淮海工学院学报（自然科学版）》2011年第3期78-81,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(30871935);江苏省高校科研成果产业化推进工程项目(JH10-50);连云港市科技发展(社会发展)项目(SH0923);江苏省海洋资源开发研究院科技开发基金资助项目(JSIMR09B10)

摘　　要：从采自连云港连岛海域的海泥中通过透明圈平板筛选法(以胶体壳聚糖为唯一碳源)筛出28株壳聚糖酶产生菌,经过复筛,选取了其中产酶能力最高的菌株为目的菌株,其酶活力达到3.89U/mL。经过形态观察、生理生化和16S rDNA鉴定,将该菌初步鉴定为交替假单胞菌(Pseudoalteromonas sp.)。Twenty-eight chitosanase-producing bacteria were isolated from mud and sea water collected by the direct plating method using 10g/L of colloidal chitosan as the sole carbon source and transparent cycle plate assay method in Lianyungang sea area.The highest chitosanase-producing strain was selected as the test strain by rehabilitation screen and measuring the activity,whose activity of chitosanase-producing was 3.89U/mL.It was identified as Pseudoalteromonas sp.based on its morphological,physiological and biochemical properties and 16S rDNA sequence analysis.

关 键 词：壳聚糖酶 筛选鉴定 交替假单胞菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]